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Erschienen in: Zeitschrift für Gerontologie und Geriatrie 8/2018

17.10.2017 | Original contribution

Combined treatment with alendronate and Drynaria rhizome extracts

Effect on fracture healing in osteoporotic rats

verfasst von: Long Chen, Ph.D, Zhou-Shan Tao, MD, Hui Chen, MD, Kailiang Zhou, MD, Dong-Sheng Zhou, Ph.D

Erschienen in: Zeitschrift für Gerontologie und Geriatrie | Ausgabe 8/2018

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Abstract

The effects of both alendronate (ALN) and Drynaria rhizome extracts (DRE) alone could promote bone healing in osteoporotic fractures but there are no reports about the combined use of ALN and DRE for promotion of bone healing of fractures in osteoporotic settings. This study investigated the effects of ALN plus DRE on fractures in osteopenic rats. Osteopenic rats underwent unilateral transverse osteotomy on the femur fixed by a sterilized Kirschner wire 2 weeks after intragastric administration of retinoic acid (80 mg/kg body weight/day). Subsequently, the animals were randomly divided into four groups: control, ALN, DRE and ALN + DRE. All rats from groups ALN, DRE and ALN + DRE received ALN (40 mg/kg, weekly), DRE (90 mg/kg/day), or both for 2, 4 and 6 weeks. The results of our study indicated that all treatment promoted fracture healing and callus formation compared to controls but ALN + DRE treatment showed significantly stronger effects than ALN or DRE alone in histological, X‑ray and biomechanical tests. These results seem to indicate that combined treatment with ALN and DRE has an additive effect on fracture healing and callus formation in osteoporotic rats.
Literatur
1.
Zurück zum Zitat Arantes HP, Gimeno SG, Chiang AY, Bilezikian JP, Lazaretticastro M (2016) Incidence of vertebral fractures in calcium and vitamin D‑supplemented postmenopausal Brazilian women with osteopenia or osteoporosis: data from arzoxifene generations trial. Arch Endocrinol Metab 60(1):54–59CrossRefPubMed Arantes HP, Gimeno SG, Chiang AY, Bilezikian JP, Lazaretticastro M (2016) Incidence of vertebral fractures in calcium and vitamin D‑supplemented postmenopausal Brazilian women with osteopenia or osteoporosis: data from arzoxifene generations trial. Arch Endocrinol Metab 60(1):54–59CrossRefPubMed
2.
Zurück zum Zitat Wright NC, Looker AC, Saag KG, Curtis JR, Delzell ES, Randall S, Dawson-Hughes B (2014) The recent prevalence of osteoporosis and low bone mass in the United States based on bone mineral density at the femoral neck or lumbar spine. J Bone Miner Res 29(11):2520–2526. https://doi.org/10.1002/jbmr.2269 CrossRefPubMed Wright NC, Looker AC, Saag KG, Curtis JR, Delzell ES, Randall S, Dawson-Hughes B (2014) The recent prevalence of osteoporosis and low bone mass in the United States based on bone mineral density at the femoral neck or lumbar spine. J Bone Miner Res 29(11):2520–2526. https://​doi.​org/​10.​1002/​jbmr.​2269 CrossRefPubMed
4.
Zurück zum Zitat Hannan EL, Magaziner J, Wang JJ, Eastwood EA, Silberzweig SB, Gilbert M, Morrison RS, McLaughlin MA, Orosz GM, Siu AL (2001) Mortality and locomotion 6 months after hospitalization for hip fracture: risk factors and risk-adjusted hospital outcomes. JAMA 285(21):2736–2742CrossRefPubMed Hannan EL, Magaziner J, Wang JJ, Eastwood EA, Silberzweig SB, Gilbert M, Morrison RS, McLaughlin MA, Orosz GM, Siu AL (2001) Mortality and locomotion 6 months after hospitalization for hip fracture: risk factors and risk-adjusted hospital outcomes. JAMA 285(21):2736–2742CrossRefPubMed
5.
Zurück zum Zitat Roux C, Wyman A, Hooven FH, Gehlbach SH, Adachi JD, Chapurlat RD, Compston JE, Cooper C, Diez-Perez A, Greenspan SL, Lacroix AZ, Netelenbos JC, Pfeilschifter J, Rossini M, Saag KG, Sambrook PN, Silverman S, Siris ES, Watts NB, Boonen S, GLOW investigators (2012) Burden of non-hip, non-vertebral fractures on quality of life in postmenopausal women: the Global Longitudinal study of Osteoporosis in Women (GLOW). Osteoporos Int 23(12):2863–2871. https://doi.org/10.1007/s00198-012-1935-8 CrossRefPubMedPubMedCentral Roux C, Wyman A, Hooven FH, Gehlbach SH, Adachi JD, Chapurlat RD, Compston JE, Cooper C, Diez-Perez A, Greenspan SL, Lacroix AZ, Netelenbos JC, Pfeilschifter J, Rossini M, Saag KG, Sambrook PN, Silverman S, Siris ES, Watts NB, Boonen S, GLOW investigators (2012) Burden of non-hip, non-vertebral fractures on quality of life in postmenopausal women: the Global Longitudinal study of Osteoporosis in Women (GLOW). Osteoporos Int 23(12):2863–2871. https://​doi.​org/​10.​1007/​s00198-012-1935-8 CrossRefPubMedPubMedCentral
6.
Zurück zum Zitat Mccann RM, Colleary G, Geddis C, Clarke SA, Jordan GR, Dickson GR (2008) Effect of osteoporosis on bone mineral density and fracture repair in a rat femoral fracture model. J Orthop Res 26(3):384–393CrossRefPubMed Mccann RM, Colleary G, Geddis C, Clarke SA, Jordan GR, Dickson GR (2008) Effect of osteoporosis on bone mineral density and fracture repair in a rat femoral fracture model. J Orthop Res 26(3):384–393CrossRefPubMed
8.
Zurück zum Zitat Reszka AA, Rodan GA (1998) Mechanisms of action of bisphosphonates. Annu Rev Pharmacol Toxicol 38(1):375–388CrossRef Reszka AA, Rodan GA (1998) Mechanisms of action of bisphosphonates. Annu Rev Pharmacol Toxicol 38(1):375–388CrossRef
11.
Zurück zum Zitat Adolphson P, Abbaszadegan H, Bodén H, Salemyr M, Henriques T (2000) Clodronate increases mineralization of callus after Colles’ fracture: a randomized, double-blind, placebo-controlled, prospective trial in 32 patients. Acta Orthop Scand 71(2):195–200CrossRefPubMed Adolphson P, Abbaszadegan H, Bodén H, Salemyr M, Henriques T (2000) Clodronate increases mineralization of callus after Colles’ fracture: a randomized, double-blind, placebo-controlled, prospective trial in 32 patients. Acta Orthop Scand 71(2):195–200CrossRefPubMed
12.
Zurück zum Zitat Li H, Miyahara T, Tezuka Y, Namba T, Suzuki T, Dowaki R, Watanabe M, Nemoto N, Tonami S, Seto H (1999) The effect of kampo formulae on bone resorption in vitro and in vivo. II. Detailed study of berberine. Biol Pharm Bull 22(4):391–396CrossRefPubMed Li H, Miyahara T, Tezuka Y, Namba T, Suzuki T, Dowaki R, Watanabe M, Nemoto N, Tonami S, Seto H (1999) The effect of kampo formulae on bone resorption in vitro and in vivo. II. Detailed study of berberine. Biol Pharm Bull 22(4):391–396CrossRefPubMed
13.
Zurück zum Zitat Jeong JC, Kang SC, Jeong CW, Kim HM, Lee YC, Chang YC, Kim CH (2003) Inhibition of drynariae rhizoma extracts on bone resorption mediated by processing of cathepsin K in cultured mouse osteoclasts. Int Immunopharmacol 3(12):1685–1697CrossRefPubMed Jeong JC, Kang SC, Jeong CW, Kim HM, Lee YC, Chang YC, Kim CH (2003) Inhibition of drynariae rhizoma extracts on bone resorption mediated by processing of cathepsin K in cultured mouse osteoclasts. Int Immunopharmacol 3(12):1685–1697CrossRefPubMed
14.
Zurück zum Zitat Liu RH, Kang X, Xu LP, Nian HL, Yang XW, Shi HT, Wang XJ (2015) Effects of the combined extracts of herba epimedii and fructus ligustri lucidi on bone mineral content and bone turnover in osteoporotic rats. BMC Complement Altern Med 15(1):1–8CrossRef Liu RH, Kang X, Xu LP, Nian HL, Yang XW, Shi HT, Wang XJ (2015) Effects of the combined extracts of herba epimedii and fructus ligustri lucidi on bone mineral content and bone turnover in osteoporotic rats. BMC Complement Altern Med 15(1):1–8CrossRef
15.
Zurück zum Zitat Ling-jie F, Ting-ting T, Yong-qiang H, Ke-rong D (2013) Long-term effects of alendronate on fracture healing and bone remodeling of femoral shaft in ovariectomized rats. Acta Pharmacol Sin 34(3):387–392CrossRef Ling-jie F, Ting-ting T, Yong-qiang H, Ke-rong D (2013) Long-term effects of alendronate on fracture healing and bone remodeling of femoral shaft in ovariectomized rats. Acta Pharmacol Sin 34(3):387–392CrossRef
16.
Zurück zum Zitat Hao Y, Ma Y, Wang X, Jin F, Ge S (2012) Short-term muscle atrophy caused by botulinum toxin-A local injection impairs fracture healing in the rat femur. J Orthop Res 30(4):574–580CrossRefPubMed Hao Y, Ma Y, Wang X, Jin F, Ge S (2012) Short-term muscle atrophy caused by botulinum toxin-A local injection impairs fracture healing in the rat femur. J Orthop Res 30(4):574–580CrossRefPubMed
18.
Zurück zum Zitat Manolagas SC (2000) Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev 21(2):115–137PubMed Manolagas SC (2000) Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev 21(2):115–137PubMed
19.
Zurück zum Zitat Dempster DW (2002) The impact of bone turnover and bone-active agents on bone quality: focus on the hip. Osteoporos Int 13(13):349–352CrossRefPubMed Dempster DW (2002) The impact of bone turnover and bone-active agents on bone quality: focus on the hip. Osteoporos Int 13(13):349–352CrossRefPubMed
21.
Zurück zum Zitat Moazzaz P, Gupta MC, Gilotra MM, Gilotra MN, Maitra S, Theerajunyaporn T, Chen JL, Reddi AH, Martin RB (2005) Estrogen-dependent actions of bone morphogenetic protein-7 on spine fusion in rats. Spine 30(15):1706–1711CrossRefPubMed Moazzaz P, Gupta MC, Gilotra MM, Gilotra MN, Maitra S, Theerajunyaporn T, Chen JL, Reddi AH, Martin RB (2005) Estrogen-dependent actions of bone morphogenetic protein-7 on spine fusion in rats. Spine 30(15):1706–1711CrossRefPubMed
22.
Zurück zum Zitat Hochberg MC, Greenspan S, Wasnich RD, Miller P, Thompson DE, Ross PD (2002) Changes in bone density and turnover explain the reductions in incidence of nonvertebral fractures that occur during treatment with antiresorptive agents. J Clin Endocrinol Metab 87(4):1586–1592CrossRefPubMed Hochberg MC, Greenspan S, Wasnich RD, Miller P, Thompson DE, Ross PD (2002) Changes in bone density and turnover explain the reductions in incidence of nonvertebral fractures that occur during treatment with antiresorptive agents. J Clin Endocrinol Metab 87(4):1586–1592CrossRefPubMed
23.
Zurück zum Zitat Wasnich RD, Miller PD (2000) Antifracture efficacy of antiresorptive agents are related to changes in bone density. J Clin Endocrinol Metab 85(1):231–236CrossRefPubMed Wasnich RD, Miller PD (2000) Antifracture efficacy of antiresorptive agents are related to changes in bone density. J Clin Endocrinol Metab 85(1):231–236CrossRefPubMed
24.
Zurück zum Zitat Wong RW, Rabie B, Bendeus M, Hägg U (2007) The effects of rhizoma curculiginis and rhizoma drynariae extracts on bones. Chin Med 2(1):1–7CrossRef Wong RW, Rabie B, Bendeus M, Hägg U (2007) The effects of rhizoma curculiginis and rhizoma drynariae extracts on bones. Chin Med 2(1):1–7CrossRef
25.
Zurück zum Zitat Jeong JC, Lee BT, Yoon CH, Kim HM, Kim CH (2005) Effects of drynariae rhizoma on the proliferation of human bone cells and the immunomodulatory activity. Pharmacol Res 51(2):125–136CrossRefPubMed Jeong JC, Lee BT, Yoon CH, Kim HM, Kim CH (2005) Effects of drynariae rhizoma on the proliferation of human bone cells and the immunomodulatory activity. Pharmacol Res 51(2):125–136CrossRefPubMed
26.
Zurück zum Zitat Chen LL, Lei LH, Ding PH, Tang Q, Wu YM (2011) Osteogenic effect of frynariae rhizoma extracts and Naringin on MC3T3-E1 cells and an induced rat alveolar bone resorption model. Arch Oral Biol 56(56):1655–1662CrossRefPubMed Chen LL, Lei LH, Ding PH, Tang Q, Wu YM (2011) Osteogenic effect of frynariae rhizoma extracts and Naringin on MC3T3-E1 cells and an induced rat alveolar bone resorption model. Arch Oral Biol 56(56):1655–1662CrossRefPubMed
27.
Zurück zum Zitat Jeong JC, Lee JW, Yoon CH, Lee YC, Chung KH, Kim MG, Kim CH (2005) Stimulative effects of drynariae rhizoma extracts on the proliferation and differentiation of osteoblastic MC3T3-E1 cells. J Ethnopharmacol 96(3):489–495CrossRefPubMed Jeong JC, Lee JW, Yoon CH, Lee YC, Chung KH, Kim MG, Kim CH (2005) Stimulative effects of drynariae rhizoma extracts on the proliferation and differentiation of osteoblastic MC3T3-E1 cells. J Ethnopharmacol 96(3):489–495CrossRefPubMed
28.
Zurück zum Zitat Schindeler A, Mcdonald MM, Bokko P, Little DG (2008) Bone remodeling during fracture repair: the cellular picture. Semin Cell Dev Biol 19(5):459–466CrossRefPubMed Schindeler A, Mcdonald MM, Bokko P, Little DG (2008) Bone remodeling during fracture repair: the cellular picture. Semin Cell Dev Biol 19(5):459–466CrossRefPubMed
29.
Zurück zum Zitat Hanna S, Jasmin L, Epari DR, Petra S, Christine E, Sarah M, Hermann B, Haas NP, Duda GN (2006) Osteoclastic activity begins early and increases over the course of bone healing. Bone 38(4):547–554CrossRef Hanna S, Jasmin L, Epari DR, Petra S, Christine E, Sarah M, Hermann B, Haas NP, Duda GN (2006) Osteoclastic activity begins early and increases over the course of bone healing. Bone 38(4):547–554CrossRef
Metadaten
Titel
Combined treatment with alendronate and Drynaria rhizome extracts
Effect on fracture healing in osteoporotic rats
verfasst von
Long Chen, Ph.D
Zhou-Shan Tao, MD
Hui Chen, MD
Kailiang Zhou, MD
Dong-Sheng Zhou, Ph.D
Publikationsdatum
17.10.2017
Verlag
Springer Medizin
Erschienen in
Zeitschrift für Gerontologie und Geriatrie / Ausgabe 8/2018
Print ISSN: 0948-6704
Elektronische ISSN: 1435-1269
DOI
https://doi.org/10.1007/s00391-017-1326-z

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