Skip to main content
SpringerLink
Log in

Biomechanical evaluation in osteoporosis: ovariectomized rat model

  • Original article
  • Published:
Clinical Rheumatology Aims and scope Submit manuscript

Abstract

The aim of our study was to investigate the effects of ovariectomy on rat femur biomechanical parameters. Bone mineral density (BMD) and histological investigation were also evaluated. Fourteen female Sprague–Dawley rats (seven ovariectomized, seven control) were used. BMD was measured by dual-energy X-ray absorbsiometry. Bone biomechanical parameters were measured in femoral midshaft with tensile test using a biomaterial testing machine and maximum load, stiffness, energy absorption capacity (structural properties), ultimate stress, ultimate strain, and elastic modulus (material properties) were calculated. Diaphyseal cortical bone thickness was measured by using histological method. The ovariectomized (OVX) rat femur’s BMD was 14% lower than control rats (p=0.006). Mean maximum load was 55% less than the control group’s (p=0.0001). Stiffness was 72% less in OVX rats (p=0.05). Femurs of rats with OVX had 32% less absorbed energy than controls (p=0.09). From the stress–strain curve ultimate stress, ultimate strain and elastic modulus was calculated. Elastic modulus was 53% less than controls (p=0.05). Ultimate stress decreased 21% in OVX rats (p=0.097). Ultimate strain was 25% less than controls in OVX rats. Cortical thickness was significantly decreased in OVX rats than in controls (p<0.05). In conclusion, femur biomechanical parameters are decreased in osteoporosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Dequeker J, Mundy GR (1998) Bone structure and function. In: Klippel J, Dieppe PA (eds) Rheumatology, vol 8–34. Mosby, London, pp 1–12

    Google Scholar 

  2. Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:175–191

    Article  PubMed  CAS  Google Scholar 

  3. Wronski TJ, Yen CF (1991) The ovariectomized rat as animal model for postmenopausal bone loss. Cells Mater Suppl 1:69–74

    Google Scholar 

  4. NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis, and Therapy (2001) Osteoporosis prevention, diagnosis, and therapy. JAMA 285:785–795

    Article  Google Scholar 

  5. Nigg BM, Herzog W (eds) (1999) Biomechanics of the musculo-skeletal system, 2nd edn. Wiley, New York, pp 50–63

  6. Peng Z, Tuukkanen J, Zhang H, Jamsa T, Vaananen HK (1994) The mechanical strength of bone in different rat models of experimental osteoporosis. Bone 15(5):523–532

    Article  PubMed  CAS  Google Scholar 

  7. Reddy KG, Stehno-Bittel L, Hamade S, Enwemeka CS (2001) The biomechanical integrity of bone in experimental diabetes. Diabetes Res Clin Pract 54:1–8

    Article  PubMed  CAS  Google Scholar 

  8. Currey JD (1988) The effect of porosity and mineral content on the Young’s modulus of elasticity of compact bone. J Biomech 21:131–139

    Article  PubMed  CAS  Google Scholar 

  9. Schafflerw MB, Burr DB (1988) Stiffness of compact bone: effects of porosity and density. J Biomech 21:13–16

    Article  Google Scholar 

  10. Burr DB (2002) Summary—osteoporosis and fracture risk: bone matrix quality session. J Musculoskelet Neuronal Interact 2(6):544–545

    Google Scholar 

  11. Faulkner KG (2000) Bone matters: are density increases necessary to reduce fracture risk? J Bone Miner Res 15:183–187

    Article  PubMed  CAS  Google Scholar 

  12. Chesnut CH III et al (2001) Reconsidering the effects of antiresorptive therapies in reducing osteoporotic fracture. J Bone Miner Res 16:2163–2172

    Article  PubMed  CAS  Google Scholar 

  13. Giavaresi G, Borsari V, Fini M (2004) Different diagnostic techniques for the assessment of cortical bone on osteoporotic animals. Biomed Pharmacother 58(9):494–499

    Article  PubMed  CAS  Google Scholar 

  14. Bagi CM, Mecham M, Weiss J, Miller SC (1993) Comparative morphometric changes in rat cortical bone following ovariectomy and/or immobilization. Bone 14(6):877–883

    Article  PubMed  CAS  Google Scholar 

  15. Lauritzen DB, Balena R, Shea M, Seedor JG, Markatos A, Le HM, Toolan BC, Myers ER, Rodan GA, Hayes WC (1993) Effects of combined prostaglandin and alendronate treatment on the histomorphometry and biomechanical properties of bone in ovariectomized rats. J Bone Miner Res 8(7):871–879

    Article  PubMed  CAS  Google Scholar 

  16. Aerssens J, Van Audekercke R, Geusens P, Schot LP, Osman AA, Dequeker J (1993) Mechanical properties, bone mineral content, and bone composition (collagen, osteocalcin, IGF-I) of the rat femur: influence of ovariectomy and nandrolone decanoate (anabolic steroid) treatment. Calcif Tissue Int 53(4):269-277

    Article  PubMed  CAS  Google Scholar 

  17. Lepola V, Jaloyaara P, Vaananen K (1994) The influence of clodronate on the torsional strength of the growing rat tibia in immobilization osteoporosis. Bone 15(3):367–371

    Article  PubMed  CAS  Google Scholar 

  18. Ferretti JL, Cointy GR, Capozza RF, Capiglioni R, Chiappe MA (2001) Analysis of biomechanical effect on bone and on the bone muscle interactions in small animal models. J Musculoskelet Neuronal Interact 1(3):263–274

    PubMed  CAS  Google Scholar 

  19. Martin RB, Boardman DL (1993) The effects of collagen fiber orientation, porosity, density and mineralization of bovine cortical bone bending properties. J Biomech 26(9):1047–1054

    Article  PubMed  CAS  Google Scholar 

  20. Han SM, Szarzanowicz TE, Ziv I (1998) Effect of ovariectomy and calcium deficiency on the ultrasound velocity, mineral density and strength in the rat femur. Clin Biomech (Bristol, Avon) 13:480–484

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Mersin University Medical School, Mersin, Turkey

    Ulku Comelekoglu, Serap Yalin & Oya Ogenler

  2. Department of Physical Medicine and Rehabilitation, Mersin University Medical School, Mersin, Turkey

    Selda Bagis

  3. Faculty of Pharmacy, Mersin University, Mersin, Turkey

    Serap Yalin

  4. Department of Radiology, Mersin University, Mersin, Turkey

    Altan Yildiz

  5. Pharmaceutical Technology, Faculty of Pharmacy, Mersin University, Mersin, Turkey

    N. Ozlen Sahin

  6. Department of Histology and Embryology, Mersin University Medical School, Mersin, Turkey

    Izzet Oguz

  7. Department of Physiology, Mersin University Medical School, Mersin, Turkey

    R. Hatungil

  8. Mersin Universitesi Eczacılık Fakültesi, Yenişehir Kampüsü, 33169, Mersin, Turkey

    Serap Yalin

Authors
  1. Ulku Comelekoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Selda Bagis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Serap Yalin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Oya Ogenler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Altan Yildiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. Ozlen Sahin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Izzet Oguz
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. R. Hatungil
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Serap Yalin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Comelekoglu, U., Bagis, S., Yalin, S. et al. Biomechanical evaluation in osteoporosis: ovariectomized rat model. Clin Rheumatol 26, 380–384 (2007). https://doi.org/10.1007/s10067-006-0367-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10067-006-0367-2

Keywords

Search

Navigation