Hoi Shan Wong, Hoi Yan Leung, Kam Ming Ko
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DOI: 10.4236/cm.2011.21001   PDF    HTML     6,241 Downloads   12,401 Views   Citations

Abstract

‘Yang-invigorating’ Chinese tonic herbs have been shown to enhance the myocardial mitochondrial ATP generation capacity in mice ex vivo. In the present study, we examined the effect of treatment with the methanol extract of ‘Yang-invigorating’ herbs on mitochondrial ATP generation capacity in H9c2 cardio-myocytes. The effect of ‘Yin-nourishing’ herbs was also investigated for comparison. The results indicated that all ‘Yang-invigorating’ Chinese tonic herbs dose-dependently enhanced the mitochondrial ATP genera-tion capacity in H9c2 cardiomyocytes. Three out of nine ‘Yin-nourishing’ herbs produced a dose-dependent stimulatory effect on ATP generation, but to lesser extents than those of Yang herbs. Results obtained from activity-directed fractionation of the three most potent ‘Yang-invigorating’ herbs suggested that the ATP-stimulating ingredients were rather water insoluble and largely resided in the butanol fraction. In con-clusion, ‘Yang-invigorating’ herbs invariably stimulated mitochondrial ATP generation capacity in H9c2 cardiomyocytes. The cell-based assay of ATP generation capacity may be used as pharmacological test for ‘Yang-invigorating’ Chinese tonic herbs.

Keywords

Yang, Yin, Chinese Medicine, ATP, Mitochondria, Cardiomyocytes

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Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	J. Geng and Z. Su, “Practical Traditional Chinese Medicine & Pharmacology. Volume 1: Basic Theories & Principles,” New World Press, Beijing, 1991, pp. 208-234. Doi:10.1146/annurev.ph.47.030185.003423
[2]	W.E. Jacobus, “Respiratory Control and the Integration of Heart High-Energy Phosphate Metabolism by Mitochondrial Creatine Kinase,” Annual Review of Physiology, Vol. 47, 1985, pp. 707-725.
[3]	K. M. Ko, D. H. F. Mak, P. Y. Chiu and M. K. T. Poon, “Pharmacological Basis of ‘Yang-Invigoration’ in Chinese Medicine,” Trends in Pharmacological Sciences., Vol. 25, No. 1, 2004, pp. 3-6. Doi:10.1016/j.tips.2003.11.002
[4]	K. M. Ko, T. Y. Y. Leon, D. H. F. Mak, P. Y. Chiu, Y. Du and M. K. T. Poon, “A Characteristic Pharmacological Action of ‘Yang-Invigorating’ Chinese Tonifying Herbs: Enahcnement of ATP-Generation Capacity,” Phytomedicine, Vol. 13, 2006, pp. 636-642. Doi:10.1016/j.phymed.2006.02.007
[5]	T. K. Yim and K. M. Ko, “Antioxidant and Immunomodulatory Activities of Chinese Tonifying Herbs,” Pharmaceutical Biology, Vol. 40, No. 5, 2002, pp. 329- 335. Doi:10.1076/phbi.40.5.329.8457
[6]	J. Hescheler, R. Meyer, S. Plant, W. Krautwurst, W. Rosenthal, G. Schultz, “Morphological, biochemical, and electrophysiological characterization of a clonal cell (H9c2 ) line from rat heart,” Circulation Research, Vol. 69, 1991, pp. 1476-1486.
[7]	H. Y. Leung, P. Y. Chiu, M. K. T. Poon and K. M. Ko, “A Yang-Invigorating Chinese Herbal Formula Enhances Mitochondrial Functional Ability and Antioxidant Capacity in Various Tissues of Male and Female Rats,” Rejuvenation Research, Vol. 8, No. 4, 2005, pp. 238-247. Doi:10.1089/rej.2005.8.238
[8]	P. Y. Chiu and K. M. Ko, “Time Dependent Enhancement in Mitochondrial Glutathione Status and ATP Generation Capacity by Schisandrin B Treatment Decreases the Susceptibility of Rat Hearts to Ischemia-Reperfusion Injury,” Biofactors, Vol. 19, No. 1-2, 2003, pp. 43-51. Doi:10.1002/biof.5520190106
[9]	Q. Chen, E. J. Vazquez, S. Moghaddas, C. L. Hoppel and E. J. Lesnefsky, “Production of Reactive Oxygen Species by Mitochondria: Central Role of Complex III,” Journal of Biological Chemistry., Vol. 278, No. 38, 2003, pp. 36027-36031. Doi:10.1074/jbc.M304854200
[10]	H. Y. Leung and K. M. Ko, “Herba Cistanches Extract Enhances Mitochondrial ATP Generation in Rat Hearts and H9c2 Cells,” Pharmaceutical Biology, Vol. 46, No. 6, 2008, pp. 418-424. Doi:10.1080/13880200802055883