Abstract
Sample integrity is one of the most important details to consider for the production of quality results in the laboratory. Many factors have the potential to adversely affect the sample: intrinsic patient characteristics (caused by the underlying malady and/or treatment, incorrect patient preparation, etc.), difficult or incorrectly performed collection of sample, correct timing of sample collection relative to drug administration, incorrect processing and transport within the laboratory—just to name a few. This chapter outlines standard common requirements with explanations as a basis for those limitations, and practical laboratory advice to attain and maintain dependable samples.
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References
Clinical and Laboratory Standards Institute (CLSI) (2008) Collection, transport and processing of blood specimens for testing plasma-based coagulation assays. Approved Guideline, 5th edn. NCCLS document H21-A5
World Health Organization (2002) Use of anticoagulants in diagnostic laboratory investigations. Document WHO/DIL/LAB/99.1 Rev 2, Geneva, 15 Jan 2002
Adcock DM, Kressin DC, Marlar RA (1997) Effect of 3.2% vs. 3.8% sodium citrate concentration on routine coagulation testing. Am J Clin Pathol 107(1):105–110
Koepke JA, Rodgers JL, Olivier MJ (1975) Pre-instrumental variables in coagulation testing. Am J Clin Pathol 64(5):591–596
Gosselin RC, Janatpour K, Larkin EC, Lee YP, Owings JT (2004) Comparison of samples obtained from 3.2% sodium citrate glass and two 3.2% sodium citrate plastic blood collection tubes used in coagulation testing. Am J Clin Pathol 122:843–848
Biron-Andreani C, Mallol C, Seguret F, Schved JF (2000) Plastic versus siliconized glass tubes: evaluation in current laboratory practice. Thromb Haemost 83:800–801
Kratz A, Stanganeli N, Van Cott EM (2006) A comparison of glass and plastic blood collection tubes for routine and specialized coagulation assays: a comprehensive study. Arch Pathol Lab Med 130(11):1600
Fiebig EW, Etzell JE, Ng VL (2005) Clinically relevant differences in prothrombin time and INR values related to blood sample collection in plastic vs. glass tubes. Am J Clin Pathol 124(6):902–909
Siegel JE, Swami VK, Glenn P, Peterson P (1998) Effect (or lack of it) of severe anemia on PT and APTT results. Am J Clin Pathol 110(1):106–110
Reneke J, Etzell J, Leslie S, Ng VL, Gottfried EL (1998) Prolonged prothrombin time and activated partial thromboplastin time due to underfilled specimen tubes with 109 mmol/L (3.2%) citrate anticoagulant. Am J Clin Pathol 109:754–757
Adcock DM, Kressin DC, Marlar RA (1998) Minimum specimen volume requirements for routine coagulation testing: dependence on citrate concentration. Am J Clin Pathol 109(5):595–599
Chuang J, Sadler MA, Witt DM (2004) Impact of evacuated collection tube fill volume and mixing on routine coagulation testing using 2.5-mL (pediatric) tubes. Chest 126:1262–1266
NCCLS (2003) Tubes and additives for venous blood specimen collection. Approved Standard, 5th edn. NCCLS document H1-A5, vol 23, no 33. Dec 2003
Contant G, Gouault-Heilmann M, Martinoli JL (1983) Heparin inactivation during blood storage: its prevention by blood collection in citric acid, theophylline, adenosine, dipyridamole—DTAD mixture. Thromb Res 31:365–374
Carroll WE, Wollitzer AO, Harris L et al (2001) The significance of platelet counts in coagulation studies. J Med 32:83–96
Barnes PW, Eby CS, Lukoszyk M (2002) Residual platelet counts in plasma prepared for routine coagulation on testing with the Beckman Coulter power processor. Lab Hematol 8:205–209
Brien WF, Schaus MR, Cooper KE, O’Keefe BT, Inwood M (1993) Lupus anticoagulant testing: effect of the platelet count on the activated partial thromboplastin time. Br J Biomed Sci 50(2):114–116
Tripodi A, Velsecchi C, Chantarangkul V, Vattaglioli T, Mannucci PM (2003) Standardization of activated protein C resistance testing: effects of residual platelets in frozen plasmas assessed by commercial and home-made methods. Br J Haematol 120:825–828
Favaloro EJ, Mohammed A, Coombs R, Mehrabani PA (1995) Filtered plasma as a potential cause of clinical misdiagnosis: inappropriate testing in a haematology laboratory. Br J Biomed Sci 52:243–248
Diatube H product insert (1995) Becton Dickinson Franklin Lakes NJ, 061196 Nov 1995
Blomback M, Konkle BA, Manco-Johnson MJ, Bremme K, Hellgren M, Kaaja R, on behalf of the ISTH SSC subcommittee on Women’s Health Issues (2007) Preanalytical conditions that affect coagulation testing, including hormonal status and therapy. J Thromb Haemost 5:855–888
Acknowledgments
In memory of my father, who stressed to his children the value of education and kind deeds, and from whom I learnt that a good day’s work can feed both your family and your mind. L. Stang.
Funding support was from the Canadian Institute of Health Research (L Mitchell Grant#114981).
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Stang, L.J., Mitchell, L.G. (2013). Specimen Requirements for the Haemostasis Laboratory. In: Monagle, P. (eds) Haemostasis. Methods in Molecular Biology, vol 992. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-339-8_4
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DOI: https://doi.org/10.1007/978-1-62703-339-8_4
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