Volumetry of Fetal Hearts using 3D Real-Time Matrix Echocardiography – In Vitro Validation Experiments and 3D Echocardiographic Studies in Fetuses

 

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Zusammenfassung

Ziel: Validierung der Real-time-3D-Matrix-Technologie (RT3D) für die Volumetrie von fetalen Herzen. Material und Methoden: In einer prospektiven Untersuchung an 50 Feten, davon 14 mit Herzfehlern, erfolgte die Bestimmung von Ventrikelvolumina mittels RT 3D im Vergleich zur 2D-Echokardiografie (2D). Anhand kleiner Phantome und Ballons von 0,5 – 20 ml erfolgte die Überprüfung der Genauigkeit von Distanz, Flächen- und Volumenbestimmungen sowie eine systematische Analyse möglicher Fehlerquellen. Ergebnisse: Inter- und Intraobserver-Variabilität sind in vitro exzellent, allerdings werden sowohl in 2D wie auch in RT 3D Phantomvolumina tendenziell zu klein bestimmt, die Abweichung beträgt –5,49 % (3D) bzw. –6,857 % (2D). In vivo ist die RT 3D der 2D hinsichtlich der Intra- und Interobserver-Variabilität überlegen (bei linksventrikulären Volumina beträgt der Variationskoeffizient 8,28 % (3D) vs. 13,96 % (2D) und der Intraklassen-Korrelations-Koeffizient 0,997 vs. 0,885). Auch in vivo werden Volumina mittels 2D kleiner als mittels RT 3D gemessen (mittlere Differenz –0,38 bis –0,94 ml). Aufgrund eingeschränkter Schallfenster oder erschwerter Endokardabgrenzung konnten in 6 / 50 Fällen keine zuverlässigen 3D-Messungen erfolgen. Schlussfolgerung: Real-time-3D ist eine zuverlässige und valide Methode zur Volumenmessung normaler und kranker fetaler Herzen und ist der 2D-Echokardiografie hinsichtlich Messgenauigkeit sowie Intra- und Interobserver-Variabilität überlegen. Sie erscheint daher als vielversprechende Methode für die korrekte Beurteilung der fetalen Herzgröße und Funktion.

Abstract

Purpose: The aim of this study was to assess the feasibility, accuracy and reliability of 3D real-time echocardiography for fetal heart volumetry. Materials and Methods: Fifty unselected and consecutive fetuses, including 14 with cardiac malformations, were scanned prospectively using real-time 3D matrix technology and 2D echocardiography to determine ventricular volumes. Small phantoms as well as modified balloons (0.5 – 20 ml) were used to assess the validity of 2D and 3D distance, area and volume calculations and to study potential sources of error during data acquisition and analysis. The data was evaluated by two blinded observers. Results: In vitro, real-time 3D and 2D underestimated the actual volumes by –5.49 % (3D) and –6.86 % (2D). The intraobserver and interobserver variability were excellent. In vivo, real-time 3D was superior to 2D with regard to intraobserver and interobserver variability (mean coefficient of variation 8.28 % (3D) versus 13.96 % (2D), and mean intraclass correlation coefficient 0.997 (3D) versus 0.885 (2D) for left ventricular volumes). Similar to in vitro, in vivo 2D volumes were calculated smaller than 3D volumes (mean difference –0.39 to –0.94 ml). The ventricular volumes and stroke volumes increased exponentially with gestation. Secondary to poor imaging windows in advanced gestation or inadequate delineation of endocardial borders in small hearts at less than 19 weeks, 3D data could not be analyzed sufficiently in 6 / 50 fetuses. Conclusion: Real-time 3D using a matrix transducer is a feasible, reliable and valid method for volume determination in the fetus beyond 19 weeks of gestation. If compared to 2D, real-time 3D echocardiography provides improved accuracy of cardiac volumetry, decreases intraobserver and interobserver variability and is a promising tool for the accurate assessment of cardiac size and function.

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Dr. Ulrike Herberg

Department of Pediatric Cardiology, University of Bonn

Adenauerallee 119

53113 Bonn

Germany

Phone: ++ 49/2 28/28 73 33 50

Fax: ++ 49/2 28/28 73 33 60

Email: ulrike.herberg@ukb.uni-bonn.de