Abstract
C-Reactive protein (CRP), the most characteristic of the ‘acute phase proteins’ (ref. 1) is thought to participate in the mediation and/or modulation of acute inflammatory processes, but its exact function is unknown. CRP has a Ca2+-dependent binding specificity for phosphorylcholine,2 the polar head group of two widely distributed lipids, lecithin (phosphatidylcholine, PC) and sphingomyelin (SM). A number of observations3–5 suggest that at least some of the biological activities of CRP depend on its interaction with phospholipids of cell membranes. In addition, interaction of CRP with PC- and SM-containing lipid dispersions6 and with PC-containing liposomes7 can activate the complement system. We report here that binding of CRP to model membranes of PC requires the incorporation into the bilayer of lysophosphatidylcholine (LPC). Thus, a disturbance of the molecular organisation of the bilayer appears to be necessary for binding of CRP. These findings provide a possible biochemical explanation for binding of CRP to damaged but not intact cell membranes3 and might be relevant to its biological function.
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Volanakis, J., Wirtz, K. Interaction of C-reactive protein with artificial phosphatidylcholine bilayers. Nature 281, 155–157 (1979). https://doi.org/10.1038/281155a0
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DOI: https://doi.org/10.1038/281155a0
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