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Low-level laser therapy in IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendon

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Abstract

This study evaluated IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendons treated with low-level laser therapy (LLLT). Sixty-five male Wistar rats were used. Sixty were submitted to a direct injury on Achilles tendon and then distributed into six groups: LASER 1 (a single LLLT application), LASER 3 (three LLLT applications), and LASER 7 (seven LLLT applications) and Sham 1, 3, and 7 (the same injury but LLLT applications were simulated). The five remaining animals were allocated at control group (no procedure performed). LLLT (780 nm) was applied with 70 mW of mean power and 17.5 J/cm2 of fluency for 10 s, once a day. The tendons were surgically removed and assessed immunohistochemically for IL-1β, COX-2, and PGE2. In comparisons with control (IL-1β: 100.5 ± 92.5 / COX-2: 180.1 ± 97.1 / PGE2: 187.8 ± 128.8) IL-1β exhibited (mean ± SD) near-normal level (p > 0.05) at LASER 3 (142.0 ± 162.4). COX-2 and PGE2 exhibited near-normal levels (p > 0.05) at LASER 3 (COX-2: 176.9 ± 75.4 / PGE2: 297.2 ± 259.6) and LASER 7 (COX-2: 259.2 ± 190.4 / PGE2: 587.1 ± 409.7). LLLT decreased Achilles tendon’s inflammatory process.

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Acknowledgments

We thank the 2011/00979-1, São Paulo Research Foundation (FAPESP) for supporting this study and Magna Aparecida Maltauro Soares by the technical support.

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Authors and Affiliations

  1. Interdisciplinary Surgical Science Program, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, 04021-001, Brazil

    Julio Fernandes de Jesus

  2. Instituto Butantan, Laboratory of Pathophysiology, São Paulo, SP, 05504-900, Brazil

    Diva Denelle Spadacci-Morena

  3. Rehabilitation Sciences Program, Universidade Nove de Julho-UNINOVE, São Paulo, SP, 02112-000, Brazil

    Nayra Deise dos Anjos Rabelo

  4. Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, 04021-001, Brazil

    Carlos Eduardo Pinfildi & Helio Plapler

  5. Irmandade da Santa Casa de Misericórdia de São Paulo-ISCMSP, São Paulo, SP, 01221-020, Brazil

    Thiago Yukio Fukuda

Authors
  1. Julio Fernandes de Jesus
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  2. Diva Denelle Spadacci-Morena
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  3. Nayra Deise dos Anjos Rabelo
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  4. Carlos Eduardo Pinfildi
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  5. Thiago Yukio Fukuda
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  6. Helio Plapler
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Correspondence to Julio Fernandes de Jesus.

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de Jesus, J.F., Spadacci-Morena, D.D., dos Anjos Rabelo, N.D. et al. Low-level laser therapy in IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendon. Lasers Med Sci 30, 153–158 (2015). https://doi.org/10.1007/s10103-014-1636-y

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  • DOI: https://doi.org/10.1007/s10103-014-1636-y

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