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The products of the “heat-shock” loci of Drosophila hydei

Correlation between locus 2-36A and the 70,000 MW “heat-shock” peptide

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Abstract

Antibody was raised against total Drosophila hydei embryonic cellular protein with a molecular weight between 65,000 and 70,000 dalton. This antiserum reacted with the 70,000 MW “heat-shock” peptide found, in 35S labelled cell extracts of “heat-shocked” D. hydei tissue culture cells or salivary glands. — The antibody was coupled to Sepharose 4B and this material was used to absorb polysomes obtained from tissue culture cells incubated at 37° C in the presence of tritiated RNA precursors. The relative concentrations of various RNA species complementary to the “heat-shock” loci 2-32A, 2-36A, and 2-48C in either bound, non-bound, or total polysomal material was then determined by in situ hybridization. The RNA species complementary to locus 2-36A was found to be enriched in the bound polysomal material.

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Authors and Affiliations

  1. Department of Genetics, Catholic University, Toernooiveld, Nijmegen, The Netherlands

    N. H. Lubsen & P. J. A. Sondermeijer

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  1. N. H. Lubsen
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  2. P. J. A. Sondermeijer
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Lubsen, N.H., Sondermeijer, P.J.A. The products of the “heat-shock” loci of Drosophila hydei . Chromosoma 66, 115–125 (1978). https://doi.org/10.1007/BF00295134

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  • DOI: https://doi.org/10.1007/BF00295134

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