Zusammenfassung
Die Kryokonservierung ermöglicht das Haltbarmachen biologischer Zellen für praktisch beliebig lange Zeit, sofern geeignete Kryoprotektive, Abkühlverfahren, hinreichend tiefe Lagertemperaturen und geeignete Wiedererwärmungstechniken angewandt werden. Sie stellt die Methode der Wahl dar, wenn Blutzellen oder hämatopoetische Progenitorzellen monate- oder jahrelang gelagert werden sollen.
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Literatur
Adler SP, Lawrence LT, Baggett J, Biro V, Sharp DE (1984) Prevention of transfusion-associated cytomegalovirus infection in very low-birthweight infants using frozen blood and donors seronegative for cytomegalovirus. Transfusion 24:333–335
Areman EM, Sacher RA, Deeg HJ (1990) Processing and storage of human bone marrow: a survey of current practices in North America. Bone Marrow Transplant 6:203–209
Armitage WJ (1986) Osmotic stress as a factor in the detrimentaleffect of glycerol on human platelets. Cryobiology 23:116–125
Ashwood-Smith MJ (1961) Preservation of mouse marrow at –79 °C with dimethyl sulphoxide. Nature (London) 190:1204–1205
Ashwood-Smith MJ (1964) Low temperature preservation of mouse lymphocytes with dimethyl sulfoxide. Blood 23:494–501
Atkins L (1962) Preservation of viable leucocytes in glycerol at –80 °C. Nature (London) 195:610–611
Bakken AM (2006) Cryopreserving human peripheral blood progenitor cells. Curr Stem Cell Res Ther 1:47-54
Bank H (1980) Granulocyte preservation circa 1980. Cryobiology 17:187–197
Barnes DWH, Loutit JF (1955) The radiation recovery factor: preservation by the Polge-Smith-Parkes technique. J Natl Cancer Inst 15:901–905
Beaujean F, Leforestier C, Mannoni P (1979) Clinical and functional studies of platelets frozen in 5 % dimethyl sulfoxide. Cryo-Lett 1:98–103
Berz D, McCormack EM, Winer ES, Colvin GA, Quesenberry PJ (2007) Cryopreservation of hematopoietic stem cells. Am J Hematol 82:463-472
Boer M De, Reijneke R, van de Griend RJ, Loos JA, Roos D (1981) Large-scale purification and cryopreservation of human monocytes. J Immun Meth 43:225–229
Brecher ME (ed) (2003) Technical manual, 14th ed. AABB, Bethesda
Broxmeyer HE, Douglas GW, Hangoc G, Cooper S, Bard J, EnglishD, Arny M, Thomas L, Boyse EA (1989) Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proc Natl Acad Sci USA 86:3828–3832
Choudhury C, Gunstone MJ (1978) Freeze preservation of platelets using hydroxyethyl starch (HES): a preliminary report. Cryobiology 15:493–501
Clark J, Pati A, McCarthy D (1991) Successful cryopreservation of human bone marrow does not require a controlled-rate freezer. Bone Marrow Transplant 7:121–125
Currie LM, et al. (1999) Enhanced circulatory parameters of human platelets cryopreserved with second-messenger effectors: an in vivo study of 16 volunteer platelet donors. Br J Haematol 105:826–831
Dankberg F, Persidsky MD (1976) A test of granulocyte membrane integrity and phagocytic function. Cryobiology 13:430–432
Davis JM, Rowley SD, Braine HG, Piantadosi S, Santos GW (1990) Clinical toxicity of cryopreserved bone marrow graft infusion. Blood 75:781–786
Dayian G, Rowe AW (1976) Cryopreservation of human platelets for transfusion. A glycerol-glucose, moderate rate cooling procedure. Cryobiology 13:1–8
Djerassi I, Farber S, Roy A, Cavins J (1966) Preparation and in vivo circulation of human platelets preserved with combined dimethylsulfoxide and dextrose. Transfusion 6:572–576
Douay L, Gorin NC, David R, Stachowiak J, Salmon C, Najman A, Duhamel G (1982) Study of granulocyte-macrophage progenitor (CFUc) preservation after slow freezing of bone marrow in the gas phase of liquid nitrogen. Exp Hematol 10:360–366
Deutsches Register für Stammzeltransplantationen (2007) http://www.drst.de/download/jb2007.pdf
Douay L, Lopez M, Gorin NC (1986) A technical bias: differences in cooling rates prevent ampoules from being a reliable index of stem cell cryopreservation in large volumes. Cryobiology 23:296–301
Eichler H, Burkhart J, Sputtek A, Wiesneth M (2005) Stellungnahme der Sektion »Transplantation und Zelltherapie« der Deutschen Gesellschaft für Transfusionsmedizin und Immunhämatologie vom 15. August 2005: Gewinnung und Langzeitlagerung von autologen und allogenen Stammzellpräparaten aus Nabelschnurblut: Indikationen und Grenzen. Transfus Med Hemother 32:274–282
Fleming KK, Hubel A (2007) Cryopreservation of hematopoietic stem cells: emerging science, technology and issues. Tranfus Med Hemother 34:268–275
Franks F (1985) Biophysics and biochemistry at low temperatures. University Press, Cambridge
Frey NV, Lazarus HM, Goldstein SC (2006) Has allogeneic stem cell cryopreservation been given the ‘cold shoulder’? An analysis of the pros and cons of using frozen versus fresh stem cell products in allogeneic stem cell transplantation. Bone Marrow Transplant 38:399– 405
Gluckman E, Thierry D, Lesage S, Traineau R, Gerotta J, Rabian C, Brossard Y, Nifterik J Van, Benbunan M (1992) Cord blood banking for human hematopoietic cell transplantation. Prog Clin Biol Res 377:591–598
Gorin NC (1992) Cryopreservation and storage of stem cells. In: Areman EM, Deeg HJ, Sacher RA (eds) Bone marrow and stem cell processing – a manual of current techniques. Davis, Philadelphia, pp 292–362
Hartmann U, Nunner B, Körber C, Rau G (1991) Where should the cooling rate be determined in an extended freezing sample? Cryobiology 28:115–130
Haugen RK (1979) Hepatitis after the transfusion of frozen red cells and washed red cells. N Engl J Med 301:393–395
Haynes LL, Tullis JL, Pyle HM, Wallach S, Sproul MT (1960) Clinical use of glycerolized frozen blood. JAMA 173:1657
Helmerhorst FM, ten Berge ML, van der Plas-van Dalen CM, Engelfriet CP, von dem Borne AEGK (1984) Platelet freezing for serological purposes with and without a cryopreservative. Vox Sang 46:318–322
Hess JR (2004) Red cell freezing and its impact on the supply chain. Transfus Med 14:1–8
Holovati JL, Hannon JL, Gyongyossy-Issa MIC, Acker JP (2009) Blood pereservation workshop: new and emerging trends in research and clinical practice. Transfus Med Rev 23:25–41
Horn P, et al. (1997) Transfusion of autologous, hydroxyethyl starch cryopreserved red blood cells in patients. Anaesth Analg 85:739–745
Huggins CE (1963) Preservation of blood by freezing with dimethyl sulfoxide and its removal by dilution and erythrocyte agglomeration. Vox Sang 8:99–100
Hunt SM, Lionetti FJ, Valeri CR, Callahan AB (1981) Cryogenic preservation of monocytes from human blood and platelet pheresis cellular residues. Blood 57:592–598
Kessinger A, Schmit-Pokorny K, Smith D, Armitage J (1990) Cryopreservation and infusion of autologous peripheral blood stem cells. Bone Marrow Transplant 5 (Suppl 1):25–27
Kim BK, Baldini MG (1986) Glycerol stress and platelet integrity. Cryobiology 23:209–213
Klein E, Toch R, Farber S, Freeman G, Fiorentino R (1956) Hemostasis in thrombocytopenic bleeding following infusion of stored, frozen platelets. Blood 11:693–698
Knight SC (1980) Preservation of leucocytes. In: Ashwood-Smith MJ, Farrant J (eds) Low temperature preservation in medicine and biology. Pitman Medical, Tunbridge Wells, pp 121–138
Knorpp CT, Merchant WR, Gikas PW, Spencer HH, Thompson NW (1967) Hydroxyethyl starch: extracellular cryophylactic agent for erythrocytes. Science 57:1312–1313
Körber C (1988) Phenomena at the advancing ice-liquid interface: solutes, particles and biological cells. Q Rev Biophys 21:229–298
Körber C, Scheiwe MW, Boutron P, Rau G (1982) The influence of hydroxyethyl starch on ice formation in aqueous solutions. Cryobiology 19:478–492
Körber C, Englich S, Rau G (1991) Intracellular ice formation: cryomicroscopical and calorimetric measurement. J Microsc 161:313–325
Körbling M, Dörken B, Ho AD, Pezzutto A, Hunstein W, Fliedner TM (1986) Autologous transplantation of blood derived haemopoietic stem cells after myeloablative therapy in a patient with Burkitt’s lymphoma. Blood 67:529–532
Krijnen HW, Kuivenhoven ACJ, De Wit JJFM (1968) The preservation of blood cells in the frozen state. Cryobiology 5:136–143
Landolt-Börnstein (1960) Zahlenwerte und Funktionen. Springer, Berlin
Langer R, Albrecht R, Hempel K, Krug S, Sputtek A, Steigerwald R, Trenkel K, Henrich HA (1994) Charakterisierung der 24-h-Überlebensrate und Lebensdauer von mittels Hydroxyethylstärke kryokonservierten Erythrozyten nach autologer Transfusion im Hund. Infusionsther Transfusionsmed 21:393–400
Law P, Alsop P, Dooley DC, Meryman HT (1983) Studies of cell separation: a comparison of the osmotic response of human lymphocytes and granulocyte-monocyte progenitor cells. Cryobiology 20:644–651
Lazarchick J, Jones TJ, Russell RJ, Hall SA (1988) Platelet antibody detection using frozen pooled human lymphocyte antigen-typed platelets as target cells. Diagn Clin Immunol 5:377–380
Lee JH, Klein HG (2005) Donor lymphocyte infusion: transfusion immunotherapy following allogeneic hematopoietic transplantation. In: Mintz PD (ed) Transfusion therapy: clinical principles and practice. AABB Press, Bethesda, pp 373–390
Lelkens CCM, Koning JG, de Kort B, Floot IBG, Noorman F (2006) Experiences with frozen Blood products in the Netherlands military. Transfus Apheresis Sci 34:289–298
Leibo SP, Farrant J, Mazur P, Hanna MG, Smith LH (1970) Effects of freezing on marrow stem cell suspensions: interactions of cooling and warming rates in the presence of PVP, sucrose, or glycerol. Cryobiology 6:315–332
Lioznov M, Dellbrügger C, Sputtek A, Fehse B, Kröger N, Zander AR (2008) Transportation and cryopreservation may impair haematopoietic stem cell function and engraftment of allogeneic PBSCs, but not BM. Bone Marrow Transplant 42:121–128
Lovelock JE (1953) The hemolysis of human red blood cells by freezing and thawing. Biochim Biophys Acta 10:414–426
Lozano M, et al. (2000) Effects of the addition of second-messenger effectors to platelet concentrates separated from whole-blood donations and stored at 4 degrees C or - 80 degrees C. Transfusion 40:527–534
Lundberg A, Yankee RA, Henderson ES, Pert JH (1967) Clinical effectiveness of blood platelets preserved by freezing. Transfusion 7:380–381
Makino S, Harada M, Akashi K, Taniguchi S, Shibuya T, Inaba S, Niho Y (1991) A simplified method for cryopreservation of peripheral blood stem cells at - 80 degrees C without rate-controlled freezing. Bone Marrow Transplant 8:239–244
Mazur P (1963) Kinetics of water loss from cells at subzero temperatures and the likelihood of intracellular freezing. J Gen Physiol 47:34–45
Mazur P, Schmidt JJ (1968) Interactions of cooling velocity, temperature, and warming velocity on the survival of frozen and thawed yeast. Cryobiology 5:1–17
Mazur P, Leibo SP, Chu EHY (1972) A two-factor hypothesis of freezing injury. Exp Cell Res 71:345–355
Meulen FW Van der, Reiss M, Stricker EAM, Elven E Van, von dem Borne AEGK (1981) Cryopreservation of human monocytes. Cryobiology 18:337–343
Meryman HT, Hornblower M (1972) A method for freezing and washing red blood cells using a high glycerol concentration. Transfusion 12:145–156
Meryman HT (1989) Frozen red cells. Transfus Med Rev 3:121–127
Miller VM (ed) (1977) Technical manual, 7 th edn. AABB; genehmigte deutsche Übersetzung ausgewählter Kapitel der 7. Aufl. (1986). Ortho Diagnostic Systems, Neckargemünd
Mollison PL, Sloviter HA (1951) Successful transfusion of previously frozen human red cells. Lancet II, 261:862–864
Pedralozzi P, et al. (2000) Transfusion of platelet concentrates cryopreserved with ThromboSol plus low-dose dimethylsulfoxide in patients with severe thrombocytopenia: a pilot study. Br J Haematol 108:653– 659
Pegg PJ (1965) The preservation of leucocytes for cytogenetic and cytochemical studies. Br J Haematol 11:586–591
Polge C, Smith AU, Parkes AS (1949) Revival of spermatozoa after vitrification and dehydratation at low temperatures. Nature (London) 164:666
Porsche AM, Körber C, Englich S, Hartmann U, Rau G (1986) Determination of the permeability of human lymphocytes with a microscope diffusion chamber. Cryobiology 23:302–316
Richter E, Fitzner E, Freund B, Hackensellner HA, Matthes G, Schürer M, Ziemer S (1983) Eine Methode zur Kryokonservierung von Thrombozyten. Folia Haematol (Leipzig) 110:725–730
Rinfret AP (1963) Some aspects of preservation of blood by rapid freeze-thaw procedures. Fed Proc 22:94–101
Rowe AW, Eyster E, Kellner A (1968) Liquid nitrogen preservation of red blood cells for transfusion: a low glycerol-rapid freeze procedure. Cryobiology 5:119–128
Rowe AW (1973) Preservation of blood by the low glycerol-rapid freeze process. In: American Association of Blood Banks (ed) Red Cell Freezing. AABB, Washington, pp 55–71
Rowley SD (1992) Techniques of bone marrow and stem cell cryopreservation and storage. In: Sacher RA, AuBuchon JP (eds) Marrow transplantation: Practical and technical aspects of stem cell reconstitution. AABB, Bethesda, pp 105–128
Rowe AW (2002) Cryopreservation of red blood cells by freezing and vitrification – some recollections and predictions. Infus Ther Transfus Med 29:25–30
Rubinstein P, Rosenfield RE, Adamson JW, Stevens CE (1993) Stored placental blood for unrelated bone marrow reconstitution. Blood 81:942–949
Rubinstein P, et al. (1998) Outcomes among 562 recipients of placentalblood transplants from unrelated donors. N Engl J Med 22:1565–1577
Schaefer UW, Beyer JH (1975) Die protektive Wirkung von Hydroxyäthylstärke bei der Kryokonservierung von Knochenmark der Maus und des Menschen. Anaesthesist 24:505–506
Scheiwe MW, Nick HE, Körber C (1982) An experimental study on the freezing of red blood cells with and without hydroxyethyl starch. Cryobiology 19:461–477
Schiffer CA, Aisner J, Wiernik PH (1978) Frozen autologous platelet transfusion for patients with leukemia. N Engl J Med 299:7–12
Schiffer CA, Aisner J, Dutcher JP (1985) Platelet cryopreservation using dimethyl sulfoxide. Ann N Y Acad Sci 459:161–169
Sloviter HA (1951) Recovery of human red blood cells after freezing. Lancet I, 261:823
Smith AU (1950) Prevention of haemolysis during freezing and thawing of red blood-cells. Lancet II, 259:910–911
Soiffer RJ (1997) Donor lymphocyte infusions: the door is open. J Clin Oncol 15:416–417
Sputtek A (2007) Cryopreservation of red cells and platelets. Methods Mol Biol 368:283-301
Sputtek A, Körber C (1991) Cryopreservation of red blood cells, platelets, lymphocytes, and stem cells. In: Fuller BJ, Grout BWW (eds) Clinical applications of cryobiology. CRC, Boca Raton, pp 95–147
Sputtek A, Sputtek R (2004) Cryopreservation in transfusion medicine and hematology. In: Fuller BJ, Lane N, Benson EE (eds) Life in the frozen state. CRC Press, Boca Raton, pp 483-504
Sputtek A, Brohm A, Classen I, Hartmann U, Körber C, Scheiwe MW, Rau G (1987) Cryopreservation of human platelets with hydroxyethyl starch in a one-step procedure. Cryo-Lett 8:216–229
Sputtek A, Körber C, Rau G (1991) Tieftemperaturkonservierung menschlicher roter Blutkörperchen mit dem Kryoprotektiv Hydroxyethylstärke – Bedeutung der HES–Modifikation. Z Klin Med 46:1567– 1570
Sputtek A, Singbartl G, Schleinzer W, Rau G (1994) In 2 min von der Tieflkühlkonserve zum Transfusionsbeginn: Das Ein-Schritt-Verfahren mit Hydroxyethylstärke. In: Mempel W, Heim MU, Schwarzfischer G, Mempel C (Hrsg) Eigenbluttransfusion. Hämatologie, Bd 3. Sympomed, München, S 50–58
Sputtek A, Singbartl G, Langer R, Schleinzer W, Henrich HA, Kühnl P (1995) Cryopreservation of red cells with the non-penetrating cryoprotectant haydroxyethyl starch. Cryo-Letters 16:283–288
Sputtek A, Gutensohn K, Hummel K, Löliger C, Kühnl P (1996) Zur Kryokonservierung von Blutstammzellen. J Lab Med 20:70–77
Sputtek A, Jetter S, Hummel K, Kühnl P (1997) Cryopreservation of peripheral blood progenitor cells: characteristics of suitable techniques. Beitr Infusionsther Transfusionsmed 34:79–83
Sputtek A, Kühnl P, Rowe AW (2007) Cryopreservation of erythrocytes, thrombocytes and lymphocytes. Transfus Med Hemother 34:262–267
Stiff PJ, Murgo JA, Zaroulis CG, DeRisi MF, Clarkson BD (1983) Unfractionated human marrow cell cryopreservation using dimethylsulfoxide and hydroxyethyl starch. Cryobiology 20:17–24
Sumida S (1974) Transfusion of blood preserved by freezing. Thieme, Stuttgart
Takahashi T, Hammett M, Cho MS (1985) Multifaceted freezing injury in human polymorphonuclear cells at high subfreezing temperatures. Cryobiology 22:215–236
Tocci A, Rezzoug F, Aitouche A, Touraine JL (1994) Comparison of fresh, cryopreserved and cultured haematopoietic stem cells from fetal liver. Bone Marrow Transplant 13:641–648
Tullis JL, Kethel MM, Pyle HM, Pennell RB, Gibson JG, Tinch RJ, Driscoll SG (1958) Studies on the in-vivo survival of glycerolized and frozen human red blood cells. JAMA 168:399
Vadhan-Raj S, et al. (1999) Enhanced retention of in vitro functional activity of platelets from recombinant human thrombopoietin-treated patients following long-term cryopreservation with a plateletpreserving solution (ThromboSol) and 2 % DMSO. Br J Haematol 104:403–411
Valeri CR, Zaroulis CG (1972) Rejuvenation and freezing of outdated stored human red cells. N Engl J Med 287:1307–1313
Valeri CR, Ragno G (2006) Cryopreservation of human blood products. Transfus Med Apheresis Sci 34:271-287
Valeri CR, et al. (2000) An experiment with glycerol frozen red cells stored at –80 °C for up to 37 years. Vox Sang 79:168–174
Valeri CR, et al. (2001) A multicenter of in vitro and in vivo values in human RBCs frozen with 40-percent (wt/vol) glycerol and stored after deglycerolization for 15 days at 4 °C in AS-3: Assessment of RBC processing in the ACP 215. Transfusion 41:933–939
Valeri RC, Ragno G, Van Houten P, Rose L, Rose M, Egozy Y, Popovsky M (2005) Automation of the glycerolization of red blood cells with the high-separation bowl in the Haemonetics ACP 215 instrument. Transfusion 45:1621-1627
Vecchione JJ, Chomicz SM, Emerson CP, Valeri CR (1981) Enumeration of previously frozen platelets using the Coulter counter, phase microscopy and the Technicon optical system. Transfusion 21:511–516
Walker RH (ed) (1993) Technical manual, 11th edn. AABB, Bethesda
Wissenschaftlicher Beirat der Bundesärztekammer und Paul-Ehrlich- Institut (2007) Richtlinien zur Gewinnung von Blut und Blutbestandteilen und zur Anwendung von Blutprodukten (Hämotherapie). Stand 2007
Woods EJ, Polook KE, Byers MA, Perry BC, Purtteman J, Heimfeld S, Gao D (2007) Cord blood stem cell preservation. Transfus Med Hemother 34:276-285
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Sputtek, A. (2010). Kryokonservierung von Blutzellen und hämatopoetischen Stammzellen. In: Kiefel, V., Mueller-Eckhardt, C. (eds) Transfusionsmedizin und Immunhämatologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12765-6_10
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