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Thromb Haemost 1985; 53(03): 404-407
DOI: 10.1055/s-0038-1661324
Original Article
Schattauer GmbH Stuttgart

Kinetics of In-111-Platelets in the Baboon: I. Isolation and Labelling of a Viable and Representative Platelet Population

H F Kotzé
The Blood Platelet Research Unit, South African Medical Research Council, and the University of the Orange Free State, Bloemfontein, South Africa
,
M G Lötter
The Blood Platelet Research Unit, South African Medical Research Council, and the University of the Orange Free State, Bloemfontein, South Africa
,
P N Badenhorst
The Blood Platelet Research Unit, South African Medical Research Council, and the University of the Orange Free State, Bloemfontein, South Africa
,
A du P Heyns
The Blood Platelet Research Unit, South African Medical Research Council, and the University of the Orange Free State, Bloemfontein, South Africa
› Author Affiliations
Further Information

Publication History

Received 04 October 1984

Accepted 22 March 1985

Publication Date:
18 July 2018 (online)

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Summary

A fully representative and viable platelet population was isolated from the blood of 15 baboons by a multiwash procedure, and labelled with In-lll-oxine. The recovery of the total platelet population in the circulation was 85% ± 9. Mean platelet life span was 146 hr ± 13. Correcting for plasma radioactivity (always less than 3.5%) did not significantly affect the estimate of platelet life span (145 hr ± 16) or recovery (85% ± 12). Platelet survival estimates, repeated at different times, were reproducible. In 5 baboons, platelets were also harvested by a single step differential centrifugation. The mean life span of a representative platelet population was significantly longer than that of platelets harvested by a single step. Recovery values of the representative and non-representative population were similar. We conclude that it may be important to harvest and label a fully representative platelet population for kinetic studies. The proposed method is simple and reproducible, and may be applied in studies in humans.

Keywords

Indium-111 - Primates - In-lll-labelled platelets - Platelet labelling technique - Blood platelets
 

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