Light, but not heavy alcohol drinking, stimulates paraoxonase by upregulating liver mRNA in rats and humans☆
Section snippets
Reagents
Culture medium Dulbecco’s modified Eagle’s medium (DMEM) (hi-glucose), heat inactivated fetal bovine serum, glutamine, and antibiotics were obtained from Life Technologies (Rockville, MD). Heparin (10,000 USP U/mL) was obtained from Elkin-Sinn (Cherry Hill, NJ). Dextran sulfate (50 kd) was obtained from Genzyme (Cambridge, MA). Diet components were from ICN Biomedicals (Costa Mesa, CA) or from Dyets (Bethlehem, PA). All other reagents and chemicals were of analytical grade.
Animals and feeding regimen
The humane use of
Protective effect of HDL-PON on the oxidative modification of LDL
Serum PON1 is tightly associated with HDL and inhibits oxidative modification of LDL. Thus, higher PON1 activity is thought to be beneficial in protecting against CAD, which is caused, in part, by high levels of oxLDL. Because the focus of this study is to understand the mechanism underlying the causal relationship between alcohol consumption and CAD, we have established an assay to measure LDL susceptibility to oxidation in various conditions. Thus, we performed copper sulfate-mediated LDL
Discussion
It is now well known that CAD is the principal cause of mortality and morbidity in developed countries, and several features of this metabolic disorder are due to the accumulation of oxLDL, the atherogenic lipoprotein, in the intimae of the arterial wall. On the other hand, HDL is antiatherogenic because of its ability to remove cholesterol from peripheral tissues to transport it to the liver for degradation, a process called reverse cholesterol transport. Another mechanism by which HDL acts as
References (69)
Alcoholic cardiomyopathy
Prog Cardiovasc Dis
(1984)- et al.
Arrhythmias and the “Holiday Heart”Alcohol-associated cardiac rhythm disorders
Am Heart J
(1978) - et al.
Relations of alcoholic beverage use to subsequent coronary artery disease hospitalization
Am J Cardiol
(1986) - et al.
Alcohol consumption, serum lipids and severity of angiographically determined coronary artery disease
Am J Cardiol
(1990) - et al.
High density lipoprotein cholesterol and alcohol consumption in a French male population
Atherosclerosis
(1983) - et al.
Comparison of plasma lipid and lipoprotein profiles in hypertensive black versus white men. Department of Veterans Affairs Cooperative Study Group on Antihypertensive Agents
Am J Cardiol
(1996) - et al.
Effects of alcohol consumption on serum lipoprotein-lipid and apolipoprotein concentrations. Results from an intervention study in healthy subjects
Atherosclerosis
(1986) - et al.
High-density lipoprotein subfractions and postheparin plasma lipases in alcoholic men before and after ethanol withdrawal
Metabolism
(1982) - et al.
Roles of omega 3 fatty acids and chronic ethanol in the regulation of plasma and liver lipids and plasma apoproteins A1 and E in rats
J Nutr
(1988) - et al.
High-density lipoproteins from human alcoholics exhibit impaired reverse cholesterol transport function
Metabolism
(2000)
Desialylation of human apolipoprotein E decreases its binding to high-density lipoprotein and its ability to deliver esterified cholesterol to the liver
Metabolism
Acetaldehyde binding increases the catabolism of rat serum low-density lipoproteins
Life Sci
Organophosphate detoxicating hydrolases in different vertebrate species
Comp Biochem Physiol
Is paraoxonase related to atherosclerosis
Chem Biol Interact
Paraoxonase prevents accumulation of lipoperoxides in low-density lipoprotein
FEBS Lett
Daily moderate alcohol consumption increases serum paraoxonase activityA diet-controlled, randomised intervention study in middle-aged men
Atherosclerosis
Spectrophotometric assays for the enzymatic hydrolysis of the active metabolites of chlorpyrifos and parathion by plasma paraoxonase/arylesterase
Anal Biochem
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Anal Biochem
Validation of a procedure for exogenous isotopic labeling of lipoprotein triglyceride with radioactive triolein
Biochim Biophys Acta
A polymorphism upstream from the human paraoxonase (PON1) gene and its association with PON1 expression
Atherosclerosis
The relations of alcohol and the cardiovascular system
Annu Rev Nutr
The effects of alcoholism on skeletal and cardiac muscle
N Engl J Med
Blood pressure and alcohol intake
Alcohol and hemorrhagic stroke. The Honolulu Heart Program
JAMA
A prospective study of moderate alcohol consumption and the risk of coronary disease and stroke in women
N Engl J Med
Alcohol consumption and carotid atherosclerosis: Evidence of dose-dependent atherogenic and antiatherogenic effects. Results from the Bruneck Study
Stroke
Alcohol consumption, diet, coronary risk factors, and prevalent coronary heart disease in men and women in the Scottish Heart Health Study
J Epidemiol Community Health
Alcohol consumption and atherosclerosis: What is the relation? Prospective results from the Bruneck Study
Stroke
Coffee, alcohol and risk of coronary heart disease among Japanese men living in Hawaii
N Engl J Med
Alcohol and exercise in myocardial infarction and sudden coronary death in men and women
Am J Epidemiol
Plasma high-density lipoprotein cholesterol level. Influence of risk factor intervention
JAMA
Alterations of lipid metabolism in healthy volunteers during long-term ethanol intake
Eur J Clin Invest
Alcohol consumption before myocardial infarction. Results from the Kaiser-Permanente epidemiologic study of myocardial infarction
Ann Intern Med
Coronary artery occlusion and alcohol intake
Br Heart J
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Supported by Grant No. R01 AA13411-01 from the National Institute on Alcohol Abuse and Alcoholism (NIAAA).