Abstract
This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barallon R, Bauer SR, Butler J, Capes-Davis A, Dirks WG, Elmore E, Furtado M, Kline MC, Kohara A, Los GV, MacLeod RA, Masters JR, Nardone M, Nardone RM, Nims RW, Price PJ, Reid YA, Shewale J, Sykes G, Steuer AF, Storts DR, Thomson J, Taraporewala Z, Alston-Roberts C, Kerrigan L (2010) Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues. Vitro Cell Dev Biol Anim 46(9):727–732
Baust JM (2007) Properties of cells and tissues influencing preservation outcome: molecular basis of preservation-induced cell death. In: Baust JM (ed) Baust JG. CRC Press, Advances in Biopreservation, pp 63–87
Baust JM, Snyder KK, Van Buskirk RG, Baust JG (2009) Changing paradigms in biopreservation. Biopreserv Biobanking 7(1):3–12
Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (2010) Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer 127(1):1–8
Dirks WG, MacLeod RA, Nakamura Y, Kohara A, Reid Y, Milch H, Drexler HG, Mizusawa H (2015) Cell line cross-contamination initiative: an interactive reference database of STR profiles covering common cancer cell lines. Int J Cancer 26(1):303–304
Freedman LP, Cockburn IM, Simcoe TS (2015a) The economics of reproducibility in preclinical research. PLoS Biol 13(6):e1002165
Freedman LP, Gibson MC, Ethier SP, Soule HR, Neve RM, Reid YA (2015b) Reproducibility: changing the policies and culture of cell line authentication. Nat Methods 12(6):493–497
Fuller BJ (2003) Gene expression in response to low temperatures in mammalian cells: a review of current ideas. Cryo Letters 24(2):95–102
Reid Y, Storts D, Riss T, Minor L (2013) Authentication of human cell lines by STR DNA profiling analysis. In: Sittampalam GS, Coussens NP, Brimacombe K, Grossman A, Arkin M, Auld D, Austin C, Baell J, Bejcek B, TDY C, Dahlin JL, Devanaryan V, Foley TL, Glicksman M, Hall MD, Hass JV, Inglese J, Iversen PW, Lal-Nag M, Li Z, Mc Gee J, Mc Manus O, Riss T, Trask OJ Jr, Weidner JR, Xia M, Xu X (eds) Assay Guidance Manual [Internet]. Eli Lilly & Company and the National Center for Advancing Translational Sciences, Bethesda (MD)
Reid YA (2011) Characterization and authentication of cancer cell lines: an overview. Methods Mol Biol 731:35–43
Snyder KK, Van Buskirk RG, Baust JM, Mathew AJ, Baust JG (2004) Biological packaging for the global cell and tissue therapy markets. Bioprocessing 3(3):39–45
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Baust, J.M., Buehring, G.C., Campbell, L. et al. Best practices in cell culture: an overview. In Vitro Cell.Dev.Biol.-Animal 53, 669–672 (2017). https://doi.org/10.1007/s11626-017-0177-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11626-017-0177-7