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Circulating extracellular vesicles in the aging process: impact of aerobic exercise

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Abstract

Our aim was to investigate transitory and delayed exercise effects on serum extracellular vesicles (EVs) in aging process. Male Wistar rats of 3-, 21-, and 26-month old were allocated into exercised and sedentary groups. The exercise protocol consisted in a daily moderate treadmill exercise (20 min daily during 2 weeks). Trunk blood was collected 1 and 18 h after the last exercise session, and circulating EVs were obtained. CD63 levels and acetylcholinesterase (AChE) activity were used as markers of exosome, a subtype of EVs. In addition, the quantification of amyloid-β (Aβ) levels and the oxidative status parameters, specifically reactive species content, superoxide dismutase (SOD) activity, and SOD1 content were evaluated. Aged rats showed reduced CD63 levels and increased AChE activity in circulating exosomes compared to young ones. Moreover, higher reactive species levels were found in circulating EVs of aged rats. Delayed exercise effects were observed on peripheral EVs, since CD63, reactive species content, and AChE activity were altered 18 h after the last exercise session. Our results suggest that the healthy aging process can modify circulating EVs profile, and exercise-induced beneficial effects may be related to its modulation on EVs.

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Acknowledgements

This work received financial support from Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (Grant #No. 476634/2013-01). Dr. I.R. Siqueira; K. Bertoldi; L.R. Cechinel; B. Schallenberger received CNPq fellowships. The authors would like to acknowledge Prof. Adriana Pohlmann for providing access to Nanosight LM10-HS Instrument.

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  1. Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Karine Bertoldi, Laura Reck Cechinel, Giana Blume Corssac, Adriane Belló-Klein, Alex Sander R. Araujo & Ionara Rodrigues Siqueira

  2. Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Bruna Schallenberger & Ionara Rodrigues Siqueira

  3. Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Samuel Davies & Irene Clemes Külkamp Guerreiro

  4. Laboratório de Fisiologia Cardiovascular e Espécies Reativas do Oxigênio, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Giana Blume Corssac, Adriane Belló-Klein & Alex Sander R. Araujo

  5. Laboratório de Neuropsicofarmacologia, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, sala 313, 90050-170, Porto Alegre, Rio Grande do Sul, Brazil

    Ionara Rodrigues Siqueira

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  1. Karine Bertoldi
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Bertoldi, K., Cechinel, L.R., Schallenberger, B. et al. Circulating extracellular vesicles in the aging process: impact of aerobic exercise. Mol Cell Biochem 440, 115–125 (2018). https://doi.org/10.1007/s11010-017-3160-4

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