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Zeitschrift für Gerontologie und Geriatrie

Erschienen in:

01.07.2016 | Originalien

Effect of teriparatide on repair of femoral metaphyseal defect in ovariectomized rats

verfasst von: Zhou-Shan Tao, Yang-Xun Lv, Wei Cui, Zheng-liang Huang, Kai-Kai Tu, Qiang Zhou, Tao Sun, Lei Yang

Erschienen in: Zeitschrift für Gerontologie und Geriatrie | Ausgabe 5/2016

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Abstract

Objective

This study aimed to investigate the effect exerted by teriparatide on the repair of femoral metaphyseal defect in ovariectomized rats.

Method

Female Sprague–Dawley rats were ovariectomized and after 3 months a critically sized defect of 3 mm in diameter—a through-hole bone defect—was drilled into each distal femur of the ovariectomized rats. The rats were injected with teriparatide (30 μg/kg) parathyroid hormone (PTH) in the peritoneum three times per week. After 4 and 8 weeks the animals were killed and the blood and bilateral femora were harvested for biochemical analysis, histopathological observation, and micro-computed tomography (CT) examination.

Results

The PTH group and control group were compared 4 and 8 weeks after surgery. PTH increased bone formation in the defect area. Moreover, PTH showed the strongest effects on bone volume per total volume, trabecular number, trabecular thickness, trabecular separation, and total fluorescence-marked new bone area. Additionally, the PTH treatment group showed inhibited serum concentrations of C-terminal telopeptide of type I collagen and enhanced expression of calcium, phosphorus, and bone alkaline phosphatase.

Conclusion

Our findings suggest a positive effect of PTH on defect healing in ovariectomized rats.

Vorheriger Artikel DEL-FINE: a new tool for assessing the delirogenic properties of drugs of relevance for European pharmacotherapy

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Titel: Effect of teriparatide on repair of femoral metaphyseal defect in ovariectomized rats
verfasst von: Zhou-Shan Tao
Yang-Xun Lv
Wei Cui
Zheng-liang Huang
Kai-Kai Tu
Qiang Zhou
Tao Sun
Lei Yang
Publikationsdatum: 01.07.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Zeitschrift für Gerontologie und Geriatrie / Ausgabe 5/2016
Print ISSN: 0948-6704
Elektronische ISSN: 1435-1269
DOI: https://doi.org/10.1007/s00391-015-0949-1

Springerpflege

Abstract

Objective

Method

Results

Conclusion

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