Abstract
Thrombin clotting time (TCT) is a coagulation assay used to diagnose congenital and acquired fibrinogen deficiency (Adcock et al., Coagulation handbook, Esoterix Coagulation, Austin, TX, 2002), as well as to identify contamination by heparin, prior to performing additional coagulation assays.
This test is based on the principle that in citrated plasma, the addition of Thrombin allows for formation of a stable clot. The time required for the formation of a stable clot is recorded in seconds and represents the actual TCT result.
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References
Clauss VA (1957) Gerinnungsphysiologische schnell methode zur bestimmun des fibrinogens. Acta Haematol 17:237–246
Flanders MM, Crist R, Rodgers GM (2003) Comparison of five thrombin time reagents. Clin Chem 49:169–172
Bockenstedt PL (1998) Laboratory methods in hemostasis. In: Loscalzo J, Schafer AI (eds) Thrombosis and hemorrhage, 2nd edn. Williams and Wilkins, Baltimore, pp 517–580
Ts’ao CH, Raymond J, Kolb T, Lo R (1976) Effects of source and concentration of thrombin, and divalent cations, on thrombin time of heparinized plasma. Am J Clin Pathol 65:206–212
Ray MJ, Perrin EJ, Smith IR, Hawson GA (1996) A proposed model to monitor heparin therapy using the concentrated thrombin time which allows standardisation of reagents and improved estimation of heparin concentrations. Blood Coag Fibrinol 7:515–521
Chan A, Black L, Ing C, Williams S, Brandao L, Walter K, Abdelhaleem M (2005) Utility of aPTT in monitoring unfractionated heparin in children. J Thromb Haemost 3, abstract OR360.
Reid TJR, Alving BM (1993) A quantitative thrombin time for determining levels of hirudin and hirulog. Thromb Haemost 70:608–616
Shore-Lesserson L, Manspeizer HE, Bolastig M, Harrington D, Vela-Cantos F, DePerio M (2000) Anticoagulation for cardiac surgery in patients receiving preoperative heparin: use of high-dose thrombin time. Anaesth Analg 90:813–818
Wang JS, Lin C, Karp RB (1994) Comparison of high-dose thrombin time with activated clotting time for monitoring of anticoagulant effects of heparin in cardiac surgical patients. Anaesth Analg 79:9–13
Love JE, Ferrell C, Chandler WL (2007) Monitoring direct thrombin inhibitors with a plasma diluted thrombin time. Thromb Haemost 98:234–242
Monagle P, Barnes C, Ignjatovic V, Furmedge J, Newall F, Chan A, De Rosa L, Hamilton S, Ragg P, Robinson S, Auldist A, Crock C, Roy N, Rowlands S (2006) Developmental haemostasis: impact for clinical haemostasis laboratories. Thromb Haemost 95:362–372
Andrew M, Paes B, Milner R, Johnston M, Mitchell L, Tollefsen DM, Powers P (1987) Development of the human coagulation system in the full-term infant. Blood 70:165–172
Andrew M, Paes B, Milner R, Jonston M, Mitchell L, Tollefsen D, Powers P (1988) Development of the human coagulation system in the healthy premature infant. Blood 72:1651–1657
Andrew M, Vegh P, Johnston M, Bowker J, Ofosu F, Mitchell L (1992) Maturation of the hemostatic system during childhood. Blood 80:1998–2005
Aronstam A, Dennis B, Friesen MN, Clark WF, Linton AL, Lindsay RM (1978) Ristocetin and the thrombin clotting time. J Clin Pathol 31:1106–1110
NCCLS (1998) Collection, transport and processing of blood specimens for coagulation testing and general performance of coagulation assays. In: Standards NCCL (ed) 3rd edn, NCCLS, 940 West Valley Road, Wayne, PA, USA.
Adcock DM, Jensen R, Johns CS, Macy PA (2002) Coagulation handbook. Esoterix Coagulation, Austin, TX
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Ignjatovic, V. (2013). Thrombin Clotting Time. In: Monagle, P. (eds) Haemostasis. Methods in Molecular Biology, vol 992. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-339-8_10
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DOI: https://doi.org/10.1007/978-1-62703-339-8_10
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