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2018 | OriginalPaper | Buchkapitel

15. Forschungsaspekte und In-vitro-Modelle

verfasst von : Martin Gauster, Michael Gruber, Birgit Hirschmugl, Carolin Schliefsteiner, Christian Wadsack

Erschienen in: Die Plazenta

Verlag: Springer Berlin Heidelberg

Zusammenfassung

Da es kein adäquates Tiermodell zur Untersuchung der humanen Plazentaentwicklung gibt, beruhen Erkenntnisse zu zellbiologischen Abläufen der Plazentaentwicklung, wie etwa Aspekte der Trophoblastdifferenzierung, aber auch immunologische und endokrine Funktionen der humanen Plazenta, zu einem wesentlichen Anteil auf In-vitro-Untersuchungen an unterschiedlichen Zell- und Gewebskulturmodellen.
Literatur
Zurück zum Zitat Baranska P et al (2005) Expression of integrins and adhesive properties of human endothelial cell line EA.hy 926. Cancer Genomics & Proteomics 270(2):265–269 Baranska P et al (2005) Expression of integrins and adhesive properties of human endothelial cell line EA.hy 926. Cancer Genomics & Proteomics 270(2):265–269
Zurück zum Zitat Ben Amara A et al (2013) Placental macrophages are impaired in chorioamnionitis, an infectious pathology of the placenta. J Immunol 191:5501–5514 CrossRef Ben Amara A et al (2013) Placental macrophages are impaired in chorioamnionitis, an infectious pathology of the placenta. J Immunol 191:5501–5514 CrossRef
Zurück zum Zitat Bilban M et al (2010) Trophoblast invasion: assessment of cellular models using gene expression signatures. Placenta 31(11):989–996 CrossRef Bilban M et al (2010) Trophoblast invasion: assessment of cellular models using gene expression signatures. Placenta 31(11):989–996 CrossRef
Zurück zum Zitat Buerki-Thurnherr T, Von Mandach U, Wick P (2012) Knocking at the door of the unborn child: engineered nanoparticles at the human placental barrier. Swiss Med Wkly 142:w13559 PubMed Buerki-Thurnherr T, Von Mandach U, Wick P (2012) Knocking at the door of the unborn child: engineered nanoparticles at the human placental barrier. Swiss Med Wkly 142:w13559 PubMed
Zurück zum Zitat Bulmer JN, Johnson PM (1984) Macrophage populations in the human placenta and amniochorion. Clin Exp Immunol 57(2):393–403 PubMedPubMedCentral Bulmer JN, Johnson PM (1984) Macrophage populations in the human placenta and amniochorion. Clin Exp Immunol 57(2):393–403 PubMedPubMedCentral
Zurück zum Zitat Burton GJ, Charnock-Jones DS, Jauniaux E (2009) Regulation of vascular growth and function in the human placenta. Reprod 138(6):895–902 CrossRef Burton GJ, Charnock-Jones DS, Jauniaux E (2009) Regulation of vascular growth and function in the human placenta. Reprod 138(6):895–902 CrossRef
Zurück zum Zitat Challier J, Schneider H, Dancis J (1976) In vitro perfusion of human placenta. v. oxygen consumption. Am J Obstet Gynecol 126(2):261–265 CrossRef Challier J, Schneider H, Dancis J (1976) In vitro perfusion of human placenta. v. oxygen consumption. Am J Obstet Gynecol 126(2):261–265 CrossRef
Zurück zum Zitat Chi J-T et al (2003) Endothelial cell diversity revealed by global expression profiling. Proc Nat Acad of Sci U.S.A 100(19):10623–10628 CrossRef Chi J-T et al (2003) Endothelial cell diversity revealed by global expression profiling. Proc Nat Acad of Sci U.S.A 100(19):10623–10628 CrossRef
Zurück zum Zitat Claise C et al (1999) Oxidized-LDL induce apoptosis in HUVEC but not in the endothelial cell line EA.hy 926. Atherosclerosis 147(1):95–104 CrossRef Claise C et al (1999) Oxidized-LDL induce apoptosis in HUVEC but not in the endothelial cell line EA.hy 926. Atherosclerosis 147(1):95–104 CrossRef
Zurück zum Zitat Dancis J et al (1973) Transfer across perfused human placenta II. Free fatty acids. Pediatr Res 7(4):192–197 CrossRef Dancis J et al (1973) Transfer across perfused human placenta II. Free fatty acids. Pediatr Res 7(4):192–197 CrossRef
Zurück zum Zitat Dekker Nitert M et al (2005) IGF-I/insulin hybrid receptors in human endothelial cells. Mol Cell Endocrinol 229(1–2):31–37 CrossRef Dekker Nitert M et al (2005) IGF-I/insulin hybrid receptors in human endothelial cells. Mol Cell Endocrinol 229(1–2):31–37 CrossRef
Zurück zum Zitat Desoye G et al (1994) Insulin receptors in syncytiotrophoblast and fetal endothelium of human placenta. Immunohistochemical evidence for developmental changes in distribution pattern. Histochem 101(4):277–285 CrossRef Desoye G et al (1994) Insulin receptors in syncytiotrophoblast and fetal endothelium of human placenta. Immunohistochemical evidence for developmental changes in distribution pattern. Histochem 101(4):277–285 CrossRef
Zurück zum Zitat Douglas GC, King BF (1989) Isolation of pure villous cytotrophoblast from term human placenta using immunomagnetic microspheres. J Immunol Methods 119(2):259–268 CrossRef Douglas GC, King BF (1989) Isolation of pure villous cytotrophoblast from term human placenta using immunomagnetic microspheres. J Immunol Methods 119(2):259–268 CrossRef
Zurück zum Zitat Frank H et al (2001) Cell culture models of human trophoblast: primary culture of trophoblast – a workshop report in cooperation with A. Placenta 22(15):107–109 CrossRef Frank H et al (2001) Cell culture models of human trophoblast: primary culture of trophoblast – a workshop report in cooperation with A. Placenta 22(15):107–109 CrossRef
Zurück zum Zitat Frank HG et al (2000) Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid. Cancer Genet and Cytogenet 116(1):16–22 CrossRef Frank HG et al (2000) Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid. Cancer Genet and Cytogenet 116(1):16–22 CrossRef
Zurück zum Zitat Genbacev O, Schubach SA, Miller RK (1992) Villous culture of first trimester human placenta-model to study extravillous trophoblast (EVT) differentiation. Placenta 13(5):439–461 CrossRef Genbacev O, Schubach SA, Miller RK (1992) Villous culture of first trimester human placenta-model to study extravillous trophoblast (EVT) differentiation. Placenta 13(5):439–461 CrossRef
Zurück zum Zitat Guilbert LJ et al (2002) Preparation and functional characterization of villous cytotrophoblasts free of syncytial fragments. Placenta 23(2–3):175–183 CrossRef Guilbert LJ et al (2002) Preparation and functional characterization of villous cytotrophoblasts free of syncytial fragments. Placenta 23(2–3):175–183 CrossRef
Zurück zum Zitat Hannan NJ et al (2010) Models for study of human embryo implantation: choice of cell lines? Biol reprod 82(2):235–245 CrossRef Hannan NJ et al (2010) Models for study of human embryo implantation: choice of cell lines? Biol reprod 82(2):235–245 CrossRef
Zurück zum Zitat Hauguel S et al (1983) Metabolism of the human placenta perfused in vitro: glucose transfer and utilization, O2 consumption, lactate and ammonia production. Pediatr res 17(9):729–732 CrossRef Hauguel S et al (1983) Metabolism of the human placenta perfused in vitro: glucose transfer and utilization, O2 consumption, lactate and ammonia production. Pediatr res 17(9):729–732 CrossRef
Zurück zum Zitat Hertz R (1959) Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc Soc Exp Biol Med 102:77–81 CrossRef Hertz R (1959) Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc Soc Exp Biol Med 102:77–81 CrossRef
Zurück zum Zitat Hiden U et al (2007) The first trimester human trophoblast cell line ACH-3P: a novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations--TNF-alpha stimulates MMP15 expression. BMC Dev Biol 7:137 CrossRef Hiden U et al (2007) The first trimester human trophoblast cell line ACH-3P: a novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations--TNF-alpha stimulates MMP15 expression. BMC Dev Biol 7:137 CrossRef
Zurück zum Zitat Hughes SE (1996) Functional characterization of the spontaneously transformed human umbilical vein endothelial cell line ECV304: use in an in vitro model of angiogenesis. Exp Cell Res 225(1):171–185 CrossRef Hughes SE (1996) Functional characterization of the spontaneously transformed human umbilical vein endothelial cell line ECV304: use in an in vitro model of angiogenesis. Exp Cell Res 225(1):171–185 CrossRef
Zurück zum Zitat Huppertz B et al (1999) Apoptosis cascade progresses during turnover of human trophoblast: analysis of villous cytotrophoblast and syncytial fragments in vitro. Lab Invest 79(12):1687–1702 PubMed Huppertz B et al (1999) Apoptosis cascade progresses during turnover of human trophoblast: analysis of villous cytotrophoblast and syncytial fragments in vitro. Lab Invest 79(12):1687–1702 PubMed
Zurück zum Zitat Hutson JR et al (2011) The human placental perfusion model: a systematic review and development of a model to predict in vivo transfer of therapeutic drugs. Clin Pharmacol Ther 90(1):67–76 CrossRef Hutson JR et al (2011) The human placental perfusion model: a systematic review and development of a model to predict in vivo transfer of therapeutic drugs. Clin Pharmacol Ther 90(1):67–76 CrossRef
Zurück zum Zitat Irvin MW et al (2014) Techniques and assays for the study of angiogenesis. Exp Bio Med 239(11):1476–1488 CrossRef Irvin MW et al (2014) Techniques and assays for the study of angiogenesis. Exp Bio Med 239(11):1476–1488 CrossRef
Zurück zum Zitat Jaffe EA et al (1973) Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Inv 52(11):2745–2756 CrossRef Jaffe EA et al (1973) Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Inv 52(11):2745–2756 CrossRef
Zurück zum Zitat Johnson EL, Chakraborty R (2012) Placental Hofbauer cells limit HIV-1 replication and potentially offset mother to child transmission (MTCT) by induction of immunoregulatory cytokines. Retrovirology 9(1):101 CrossRef Johnson EL, Chakraborty R (2012) Placental Hofbauer cells limit HIV-1 replication and potentially offset mother to child transmission (MTCT) by induction of immunoregulatory cytokines. Retrovirology 9(1):101 CrossRef
Zurück zum Zitat Kawata M, Parnes JR, Herzenberg LA (1984) Transcriptional control of HLA-A, B, C antigen in human placental cytotrophoblast isolated using trophoblast- and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter. J Exp Med 160(3):633–651 CrossRef Kawata M, Parnes JR, Herzenberg LA (1984) Transcriptional control of HLA-A, B, C antigen in human placental cytotrophoblast isolated using trophoblast- and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter. J Exp Med 160(3):633–651 CrossRef
Zurück zum Zitat Kim J et al (2008) Involvement of Hofbauer cells and maternal T cells in villitis of unknown etiology. Histopathology 52(4):457–464 CrossRef Kim J et al (2008) Involvement of Hofbauer cells and maternal T cells in villitis of unknown etiology. Histopathology 52(4):457–464 CrossRef
Zurück zum Zitat King A, Thomas L, Bischof P (2000) Cell culture models of trophoblast II: trophoblast cell lines – a workshop report. Placenta 21 Suppl A: 113–119 CrossRef King A, Thomas L, Bischof P (2000) Cell culture models of trophoblast II: trophoblast cell lines – a workshop report. Placenta 21 Suppl A: 113–119 CrossRef
Zurück zum Zitat Kliman HJ et al (1986) Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinol 118(4):1567–1582 CrossRef Kliman HJ et al (1986) Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinol 118(4):1567–1582 CrossRef
Zurück zum Zitat Lang I et al (2001) Differential mitogenic responses of human macrovascular and microvascular endothelial cells to cytokines underline their phenotypic heterogeneity. Cell Prolif 34(3):143–155 CrossRef Lang I et al (2001) Differential mitogenic responses of human macrovascular and microvascular endothelial cells to cytokines underline their phenotypic heterogeneity. Cell Prolif 34(3):143–155 CrossRef
Zurück zum Zitat Lang I et al (2003) Heterogeneity of microvascular endothelial cells isolated from human term placenta and macrovascular umbilical vein endothelial cells. Eur J Cell Biol 82(4):163–173 CrossRef Lang I et al (2003) Heterogeneity of microvascular endothelial cells isolated from human term placenta and macrovascular umbilical vein endothelial cells. Eur J Cell Biol 82(4):163–173 CrossRef
Zurück zum Zitat Lang I et al (2008) Human fetal placental endothelial cells have a mature arterial and a juvenile venous phenotype with adipogenic and osteogenic differentiation potential. Differ 76(10):1031–1043 CrossRef Lang I et al (2008) Human fetal placental endothelial cells have a mature arterial and a juvenile venous phenotype with adipogenic and osteogenic differentiation potential. Differ 76(10):1031–1043 CrossRef
Zurück zum Zitat Lewis RM et al (2013) Review: modelling placental amino acid transfer – from transporters to placental function. Placenta 34:S46–S51 CrossRef Lewis RM et al (2013) Review: modelling placental amino acid transfer – from transporters to placental function. Placenta 34:S46–S51 CrossRef
Zurück zum Zitat Loegl J et al (2016) Hofbauer cells of M2a, M2b and M2c polarization may regulate feto-placental angiogenesis. Reprod 152(5):447–455 CrossRef Loegl J et al (2016) Hofbauer cells of M2a, M2b and M2c polarization may regulate feto-placental angiogenesis. Reprod 152(5):447–455 CrossRef
Zurück zum Zitat Loke Y, King A, Burrows T (1995) Decidua in human implantation. Hum Reprod 10 Suppl 2: 14–21 Loke Y, King A, Burrows T (1995) Decidua in human implantation. Hum Reprod 10 Suppl 2: 14–21
Zurück zum Zitat Manyonda IT, Whitley GSJ, Cartwright JE (2001) Trophoblast cell lines: a response to the workshop report by King et al. (Placenta [2000], 21 Suppl A, Trophoblast Research, 14: S113-S119). Placenta 22(2–3): 262–263 CrossRef Manyonda IT, Whitley GSJ, Cartwright JE (2001) Trophoblast cell lines: a response to the workshop report by King et al. (Placenta [2000], 21 Suppl A, Trophoblast Research, 14: S113-S119). Placenta 22(2–3): 262–263 CrossRef
Zurück zum Zitat Mathiesen L et al (2010) Quality assessment of a placental perfusion protocol. Reprod Toxicol 30(1):138–146 CrossRef Mathiesen L et al (2010) Quality assessment of a placental perfusion protocol. Reprod Toxicol 30(1):138–146 CrossRef
Zurück zum Zitat Matsubara S et al (2003) Hofbauer cell activation and its increased glucose-6-phosphate dehydrogenase activity in second trimester-spontaneous abortion: an ultrastructural dual staining enzyme-cytochemical study. Am J Reprod Immunol 49(4):202–209 CrossRef Matsubara S et al (2003) Hofbauer cell activation and its increased glucose-6-phosphate dehydrogenase activity in second trimester-spontaneous abortion: an ultrastructural dual staining enzyme-cytochemical study. Am J Reprod Immunol 49(4):202–209 CrossRef
Zurück zum Zitat Miller RK et al (2005) Human placental explants in culture: approaches and assessments. Placenta 26(6):439–448 CrossRef Miller RK et al (2005) Human placental explants in culture: approaches and assessments. Placenta 26(6):439–448 CrossRef
Zurück zum Zitat Moskalewski S, Czarnik Z, Ptak W (1975) Demonstration of cells with IgG Receptor in Human Placenta. Biol. Neonate 26(3–4):268–273 CrossRef Moskalewski S, Czarnik Z, Ptak W (1975) Demonstration of cells with IgG Receptor in Human Placenta. Biol. Neonate 26(3–4):268–273 CrossRef
Zurück zum Zitat Narahara H, Nishioka Y, Johnston JM (1993) Secretion of platelet-activating factor acetylhydrolase by human decidual macrophages. J clin endocrinol metab 77(5):1258–1262 PubMed Narahara H, Nishioka Y, Johnston JM (1993) Secretion of platelet-activating factor acetylhydrolase by human decidual macrophages. J clin endocrinol metab 77(5):1258–1262 PubMed
Zurück zum Zitat Narahara H et al (2003) Platelet-activating factor inhibits the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages. J clin endocrinol metab 88(12):6029–6033 CrossRef Narahara H et al (2003) Platelet-activating factor inhibits the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages. J clin endocrinol metab 88(12):6029–6033 CrossRef
Zurück zum Zitat Nikolov SD, Schiebler TH (1981) Endothelial cells in the blood vessels of mature human placental villi. Acta Anat 110(4):338–344 CrossRef Nikolov SD, Schiebler TH (1981) Endothelial cells in the blood vessels of mature human placental villi. Acta Anat 110(4):338–344 CrossRef
Zurück zum Zitat Novakovic B et al (2011) Wide-ranging DNA methylation differences of primary trophoblast cell populations and derived cell lines: implications and opportunities for understanding trophoblast function. Mol Hum Reprod 17(6):344–353 CrossRef Novakovic B et al (2011) Wide-ranging DNA methylation differences of primary trophoblast cell populations and derived cell lines: implications and opportunities for understanding trophoblast function. Mol Hum Reprod 17(6):344–353 CrossRef
Zurück zum Zitat Orendi K et al (2011) Placental and trophoblastic in vitro models to study preventive and therapeutic agents for preeclampsia. Placenta 32:49–54 CrossRef Orendi K et al (2011) Placental and trophoblastic in vitro models to study preventive and therapeutic agents for preeclampsia. Placenta 32:49–54 CrossRef
Zurück zum Zitat Panigel M (1962) Placental perfusion experiments. Am J Obstet Gynecol 84(11):1664–1683 CrossRef Panigel M (1962) Placental perfusion experiments. Am J Obstet Gynecol 84(11):1664–1683 CrossRef
Zurück zum Zitat Pattillo RA, Gey GO (1968) The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res 28(7):1231–1236 PubMed Pattillo RA, Gey GO (1968) The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res 28(7):1231–1236 PubMed
Zurück zum Zitat Perazzolo S et al (2016) The influence of placental metabolism on fatty acid transfer to the fetus. J Lipid Res 58(2): jlr.P072355 Perazzolo S et al (2016) The influence of placental metabolism on fatty acid transfer to the fetus. J Lipid Res 58(2): jlr.P072355
Zurück zum Zitat Schneider H, Panigel M, Dancis J (1972) Transfer across the perfused human placenta of antipyrine, sodium and leucine. Am J Obstet Gynecol 114(6):822–828 CrossRef Schneider H, Panigel M, Dancis J (1972) Transfer across the perfused human placenta of antipyrine, sodium and leucine. Am J Obstet Gynecol 114(6):822–828 CrossRef
Zurück zum Zitat Schneider H, Mohlen K, Dancis J (1979) Transfer of amino acids across the in vitro perfused human placenta. Pediatr Res 4(1):236–240 CrossRef Schneider H, Mohlen K, Dancis J (1979) Transfer of amino acids across the in vitro perfused human placenta. Pediatr Res 4(1):236–240 CrossRef
Zurück zum Zitat Seval Y, Korgun ET, Demir R (2007) Hofbauer cells in early human placenta: possible implications in vasculogenesis and angiogenesis. Placenta 28(8–9):841–845 CrossRef Seval Y, Korgun ET, Demir R (2007) Hofbauer cells in early human placenta: possible implications in vasculogenesis and angiogenesis. Placenta 28(8–9):841–845 CrossRef
Zurück zum Zitat Shiverick K et al (2001) Cell culture models of human trophoblast II: trophoblast cell lines--a workshop report. Placenta 22 Suppl A: 104–106 CrossRef Shiverick K et al (2001) Cell culture models of human trophoblast II: trophoblast cell lines--a workshop report. Placenta 22 Suppl A: 104–106 CrossRef
Zurück zum Zitat Siwetz M et al (2016) TNF-α alters the inflammatory secretion profile of human first trimester placenta. Lab Invest 96(4):428–438 CrossRef Siwetz M et al (2016) TNF-α alters the inflammatory secretion profile of human first trimester placenta. Lab Invest 96(4):428–438 CrossRef
Zurück zum Zitat Sutton LN, Mason DY, Redman CW (1989) Isolation and characterization of human fetal macrophages from placenta. Clin Exp Immunol 78(3):437–443 PubMedPubMedCentral Sutton LN, Mason DY, Redman CW (1989) Isolation and characterization of human fetal macrophages from placenta. Clin Exp Immunol 78(3):437–443 PubMedPubMedCentral
Zurück zum Zitat Svensson-Arvelund J et al (2015) The human fetal placenta promotes tolerance against the semiallogeneic fetus by inducing regulatory T cells and homeostatic M2 macrophages. J Immunol 194:1534–1544 CrossRef Svensson-Arvelund J et al (2015) The human fetal placenta promotes tolerance against the semiallogeneic fetus by inducing regulatory T cells and homeostatic M2 macrophages. J Immunol 194:1534–1544 CrossRef
Zurück zum Zitat Tang Z et al (2011) Isolation of Hofbauer cells from human term placentas with high yield and purity. Am J Reprod Immunol 66(4):336–348 CrossRef Tang Z et al (2011) Isolation of Hofbauer cells from human term placentas with high yield and purity. Am J Reprod Immunol 66(4):336–348 CrossRef
Zurück zum Zitat Tarrade A et al (2001) Characterization of human villous and extravillous trophoblasts Isolated from first trimester placenta. Lab Invest 81(9):1199–1211 CrossRef Tarrade A et al (2001) Characterization of human villous and extravillous trophoblasts Isolated from first trimester placenta. Lab Invest 81(9):1199–1211 CrossRef
Zurück zum Zitat Uren S, Boyle W (1985) Isolation of macrophages from human placenta. J Immunol Methods 78(1):25–34 CrossRef Uren S, Boyle W (1985) Isolation of macrophages from human placenta. J Immunol Methods 78(1):25–34 CrossRef
Zurück zum Zitat Vince GS et al (1990) Flow cytometric characterisation of cell populations in human pregnancy decidua and isolation of decidual macrophages. J Immunol Methods 132(2):181–189 CrossRef Vince GS et al (1990) Flow cytometric characterisation of cell populations in human pregnancy decidua and isolation of decidual macrophages. J Immunol Methods 132(2):181–189 CrossRef
Zurück zum Zitat Wetzka B, Clark DE, Chamock-Jones DS et al (1997) Isolation of macrophages (Hofbauer cells) from human term placenta and their prostaglandin E2 and thromboxane production. Hum Reprod 12(4):847–852 CrossRef Wetzka B, Clark DE, Chamock-Jones DS et al (1997) Isolation of macrophages (Hofbauer cells) from human term placenta and their prostaglandin E2 and thromboxane production. Hum Reprod 12(4):847–852 CrossRef
Zurück zum Zitat Wilson CB, Haas JE, Weaver WM (1983) Isolation, purification and characteristics of mononuclear phagocytes from human placentas. J Immunol methods 56(3):305–317 CrossRef Wilson CB, Haas JE, Weaver WM (1983) Isolation, purification and characteristics of mononuclear phagocytes from human placentas. J Immunol methods 56(3):305–317 CrossRef
Zurück zum Zitat Wood GW (1980) Mononuclear phagocytes in the human placenta. Placenta 1(2):113–123 CrossRef Wood GW (1980) Mononuclear phagocytes in the human placenta. Placenta 1(2):113–123 CrossRef
Zurück zum Zitat Zaccheo D et al (1989) Isolation and characterization of Hofbauer cells from human placental villi. Archives Gynecol Obstet 246(4):189–200 CrossRef Zaccheo D et al (1989) Isolation and characterization of Hofbauer cells from human placental villi. Archives Gynecol Obstet 246(4):189–200 CrossRef
Metadaten
Titel
Forschungsaspekte und In-vitro-Modelle
verfasst von
Martin Gauster
Michael Gruber
Birgit Hirschmugl
Carolin Schliefsteiner
Christian Wadsack
Copyright-Jahr
2018
Verlag
Springer Berlin Heidelberg
DOI
https://doi.org/10.1007/978-3-662-55622-1_15